Siderophores are organic chelators produced by microorganisms to fulfil their iron requirements. Siderophore‐promoted dissolution of iron‐bearing minerals has been clearly documented for some siderophores, but few studies have addressed metabolizing siderophore‐producing bacteria. We investigated iron acquisition from clays by fluorescent Pseudomonads, bacteria that are ubiquitous in the environment. We focused on the interactions between smectite and Pseudomonas aeruginosa, a bacterium producing two structurally different siderophores: pyoverdine and pyochelin. The presence of smectite in iron‐limited growth media promoted planktonic growth of P. aeruginosa and biofilm surrounding the smectite aggregates. Chemical analysis of the culture media indicated increases in the dissolved silicon, iron and aluminium concentrations following smectite supplementation. The use of P. aeruginosa mutants unable to produce either one or both of the two siderophores indicated that pyoverdine, the siderophore with the higher affinity for iron, was involved in iron and aluminium solubilization by the wild‐type strain. However, in the absence of pyoverdine, pyochelin was also able to solubilize iron but with a twofold lower efficiency. In conclusion, pyoverdine and pyochelin, two structurally different siderophores, can solubilize structural iron from smectite and thereby make it available for bacterial growth.

中文翻译：

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萤光假单胞菌（Pseudomonas）的铁载体促进绿土的溶解。

铁载体是由微生物产生的满足其铁需求的有机螯合剂。对于某些铁载体，铁载体促进了含铁矿物质的溶解已被明确记录，但很少有研究涉及代谢产生铁载体的细菌。我们研究了荧光假单胞菌（一种在环境中普遍存在的细菌）从粘土中获取铁的方法。我们专注于绿土之间的相互作用P假单胞菌铜绿假单胞菌，产两种结构不同的铁载体细菌：pyoverdine和pyochelin。铁限制生长培养基中蒙脱石的存在促进了P的浮游生长。铜绿蒙脱石聚集体周围的生物膜。培养基的化学分析表明，蒙脱石补充后溶解的硅，铁和铝浓度增加。采用P 。不能产生两个铁载体之一或两者的铜绿假单胞菌突变体表明，对铁具有较高亲和力的铁载体吡over定参与了野生型菌株对铁和铝的增溶作用。但是，在没有吡啶酮的情况下，秋水仙素也能够溶解铁，但效率低两倍。综上所述，结构上不同的两种铁卟啉和pyochelin可以溶解蒙脱石中的结构铁，从而使其可用于细菌生长。