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Osteochondral tissue regenerated via a strategy by stacking pre-differentiated BMSC sheet on fibrous mesh in a gradient.
Biomedical Materials ( IF 3.9 ) Pub Date : 2019-11-08 , DOI: 10.1088/1748-605x/ab49e2
Le Jin 1 , Wenwen Zhao , Bo Ren , Lei Li , Xiaoqing Hu , Xin Zhang , Qing Cai , Yingfang Ao , Xiaoping Yang
Affiliation  

The reconstruction of osteochondral tissue remains a challenging task in clinical therapy because of its heterogeneous structure. The best way to face the challenge is to develop a biomimetic construct to mimic the multilayered gradient from cartilage, to calcified cartilage and subchondral bone. In this study, bone marrow mesenchymal stromal cells (BMSCs) were cultured on electrospun fibrous meshes and cell sheets were incubated. The fibrous meshes were composed of 50% poly(L-lactide) and 50% gelatin, displaying excellent biocompatibility, cell affinity and degradability. Differentiation of BMSC sheets on fibrous meshes was induced chondrogenically or osteogenically. In particular, the BMSC sheets were able to be efficiently induced differentiating towards calcified cartilage by using a 1:1 (v/v) mixed medium of chondrogenic and osteogenic inductive media. Thus, a gradient 3D construct was built by stacking the differently pre-differentiated cell/mesh complexes layer by layer from top to bottom to mimic the cartilage-to-bone transition. With this gradient construct being implanted in the rabbit knee osteochondral defect, it was confirmed that it could promote the tissue regeneration with intact cartilage layer formation in comparison with the multilayered construct without a gradient. The strategy of using properly pre-differentiated BMSC sheet on fibrous mesh to build the osteochondral interface was thus suggested as being feasible and effective in mimicking its hierarchical complexity, and favored the repairing of injured joint cartilage.

中文翻译:

通过将预分化的BMSC片材以梯度方式堆叠在纤维网上,通过一种策略再生骨软骨组织。

骨软骨组织的异质结构在临床治疗中仍然是一项艰巨的任务。应对挑战的最佳方法是开发一种仿生构造,以模仿从软骨到钙化软骨和软骨下骨的多层梯度。在这项研究中,将骨髓间充质基质细胞(BMSC)培养在电纺纤维网上,并孵育细胞片。纤维网由50%的聚(L-丙交酯)和50%的明胶组成,具有出色的生物相容性,细胞亲和力和降解性。BMSC片在纤维网上的分化是软骨形成或成骨诱导的。特别地,通过使用1:1,能够有效地诱导BMSC片向钙化软骨的分化。1(v / v)软骨和成骨诱导性培养基的混合培养基。因此,通过从上到下逐层堆叠不同的预分化细胞/网状复合物以模拟软骨到骨的过渡,构建了梯度3D构造。将该梯度构建体植入兔膝骨软骨缺损中,证实了与没有梯度的多层构建体相比,它可以促进具有完整软骨层形成的组织再生。因此,在纤维网上使用适当的预分化BMSC片材来建立骨软骨界面的策略被认为是可行且有效的,以模仿其层次结构的复杂性,并有利于修复受损的关节软骨。通过从上到下逐层堆叠不同的预分化细胞/网状复合物,以模拟软骨向骨骼的过渡,构建了梯度3D构建体。将该梯度构建体植入兔膝骨软骨缺损中,证实了与没有梯度的多层构建体相比,它可以促进具有完整软骨层形成的组织再生。因此,在纤维网上使用适当的预分化BMSC片材来建立骨软骨界面的策略被认为是可行且有效的,以模仿其层次结构的复杂性,并有利于修复受损的关节软骨。通过从上到下逐层堆叠不同的预分化细胞/网状复合物,以模拟软骨向骨骼的过渡,构建了梯度3D构建体。将该梯度构建体植入兔膝骨软骨缺损中,证实了与没有梯度的多层构建体相比,它可以促进具有完整软骨层形成的组织再生。因此,在纤维网上使用适当的预分化BMSC片材来建立骨软骨界面的策略被认为是可行且有效的,以模仿其层次结构的复杂性,并有利于修复受损的关节软骨。将该梯度构建体植入兔膝骨软骨缺损中,证实了与没有梯度的多层构建体相比,它可以促进具有完整软骨层形成的组织再生。因此,在纤维网上使用适当的预分化BMSC片材来建立骨软骨界面的策略被认为是可行且有效的,以模仿其层次结构的复杂性,并有利于修复受损的关节软骨。将该梯度构建体植入兔膝骨软骨缺损中,证实了与没有梯度的多层构建体相比,它可以促进具有完整软骨层形成的组织再生。因此,在纤维网上使用适当的预分化BMSC片材来建立骨软骨界面的策略被认为是可行且有效的,以模仿其层次结构的复杂性,并有利于修复受损的关节软骨。
更新日期:2019-11-01
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