The focus of significance in neuronal repair strategies is the design of scaffold systems capable of promoting neuronal regeneration and directional guidance via provision of a biomimetic environment resemblance of native neural tissue. The purpose of this study was to synthesize triple-cue electrospun aligned nanofibrous films (physical cue) of poly(3-hyroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) blended with magnesium-oleate (MgOl) (chemical cue) and N-acetyl-L-cysteine (NAC) (therapeutic cue) with potential incorporation into hollow nerve guidance conduits for an enhanced regenerative strategy. A Box-Behnken experimental design of 15 formulations, were analysed for crystallinity, textural properties and in vitro water-uptake, erosion, NAC-release and PC12 cell viability. Nucleating effects of MgOl provided tuning of PHBV electrospinning-induced crystallinity and mechanical properties. Tensile strengths and deformation moduli of ±12 MPa and ±7 MP, respectively, were attainable, thereby matching native nerve mechanics. Crystallinity changes ascribed differing release kinetics to NAC over 30 d: diffusion-based (42%-58% crystallinity with 33%-47% fractional release) and polymer-relaxational (59%-65% crystallinity with 60%-82% fractional release). The synergistic activity of MgOl and NAC increased PC12 proliferation by 32.6% compared to the control. MgOl produced dual actions as non-toxic plasticiser and PC12 cell proliferation-promoter via nucleation and neurotrophic-like effects, respectively. Controlled release of NAC imparted neuro-protectant effects on PC12 cells and promoted neurite extension, thus, making electrospun PHBV-MgOl nanofibrous films a versatile and promising approach for axonal guidance in peripheral nerve repair strategies.

中文翻译：

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用于神经支持的PHBV-油酸镁定向纳米纤维的设计和表征。

在神经元修复策略中，重要的焦点是通过提供与天然神经组织类似的仿生环境，能够促进神经元再生和定向指导的支架系统的设计。这项研究的目的是合成与油酸镁（MgOl）（化学提示）混合的聚（3-羟基丁酸-co-3-羟基戊酸）（PHBV）的三提示电纺取向纳米纤维膜（物理提示）。 N-乙酰基-L-半胱氨酸（NAC）（治疗提示），有可能整合到空心神经引导导管中，以增强再生策略。对15种配方的Box-Behnken实验设计进行了结晶度，质地特性和体外吸水率，侵蚀，NAC释放和PC12细胞活力的分析。MgO1的成核作用可调节PHBV电纺丝诱导的结晶度和机械性能。可获得分别为±12 MPa和±7 MP的拉伸强度和变形模量，从而与天然神经力学相匹配。结晶度变化归因于30天内NAC的释放动力学不同：基于扩散的（42％-58％的结晶度，具有33％-47％的分数释放）和聚合物松弛（59％-65％的结晶度，具有60％-82％的分数释放） ）。与对照相比，MgOl和NAC的协同活性使PC12增殖增加了32.6％。MgOl通过成核作用和类似神经营养的作用分别产生了无毒增塑剂和PC12细胞增殖促进剂的双重作用。NAC的控制释放对PC12细胞具有神经保护作用，并促进了神经突的延伸，因此，