With the aid of extrusion-based biofabrication strategies, neural stem/progenitor cell-laden hydrogel structures can be fabricated for use in neural research. Extrusion-based strategies can be altered in order to fulfill various requirements. In this study, mouse neural progenitor cell (NE-4C) behaviors in multiple extrusion-based fabricated microenvironments were investigated. Extrusion-based bioprinted cell-laden structures and coaxially extruded core-shell cell fibers were successfully fabricated. Cell distribution and morphology were observed in different structures with scanning electron microscopy (SEM). Genes and proteins related to cell differentiation were examined using quantitative polymerase chain reaction (qPCR) and western blot (WB). The results show that compared with NE-4Cs cultured in petri dishes, the abundance of nestin was 6.28 ± 1.38 times higher in bioprinted structures and the abundances of Tuj-1 and GFAP were 3.14 ± 1.38 and 2.11 ± 0.21 times higher in cell fibers, respectively, indicating that NE-4Cs showed stronger differentiation tendency in cell fibers and weaker tendency in printed structures. This study may provide guidance in selecting fabrication strategies for use in neural research.

中文翻译：

---

在基于挤压的体外水凝胶模型中神经祖细胞行为的比较研究。

借助基于挤压的生物制造策略，可以制造神经干/祖细胞负载的水凝胶结构，用于神经研究。可以更改基于挤出的策略，以满足各种要求。在这项研究中，研究了小鼠神经祖细胞（NE-4C）在多个基于挤压成型的微环境中的行为。成功地制造了基于挤出的生物印刷的载有细胞的结构和同轴挤出的核壳细胞纤维。用扫描电子显微镜（SEM）观察到不同结构的细胞分布和形态。使用定量聚合酶链反应（qPCR）和蛋白质印迹（WB）检查与细胞分化相关的基因和蛋白质。结果表明，与培养皿中培养的NE-4C相比，巢蛋白的丰度为6。生物印染结构高28±1.38倍，细胞纤维中Tuj-1和GFAP的丰度分别高3.14±1.38和2.11±0.21倍，表明NE-4Cs在细胞纤维中表现出较强的分化趋势，而在印刷中则较弱结构。这项研究可为选择用于神经研究的制造策略提供指导。