The objective of this study was to develop a novel porous thin poly(D,L-lactide) (PLA) film as a tissue-engineering scaffold for keratinocytes used for the replacement of damaged skin. Poly(D,l-lactic acid)/poly(ethylene glycol) (PEG: Mw 6000 or 15 000) blend films were formed by a spin coating technique. The properties and structures of these blend films were investigated. PDLA/PEG (6000) blend films were modified by microfibrillar collagen after water incubation to increase hydrophilicity and improve keratinocyte adhesion. Primary keratinocytes were seeded on PLA films, cultivated for 9 d and transplanted to rats with a model skin defect wound. The wound's healing after keratinocyte transplantation was assayed with histological and immunochemical methods. It was found that skin damage recovery was the most effective after transplantation of keratinocytes on porous PLA film modified with collagen.

中文翻译：

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聚（D，L-丙交酯）/ PEG混合膜，用于角质形成细胞培养和皮肤重建。

这项研究的目的是开发一种新型的多孔聚（D，L-丙交酯）薄膜（PLA），作为角质形成细胞的组织工程支架，用于替换受损的皮肤。通过旋涂技术形成聚（D，1-乳酸）/聚（乙二醇）（PEG：Mw 6000或15000）共混膜。研究了这些共混膜的性质和结构。水温孵育后，用微纤维胶原蛋白修饰PDLA / PEG（6000）共混膜，以增加亲水性并改善角质形成细胞的粘附。将原代角质形成细胞接种在PLA膜上，培养9天，然后移植到具有模型性皮肤缺损伤口的大鼠中。用组织学和免疫化学方法测定角质形成细胞移植后的伤口愈合。