The aim of this study was to verify fluorescence in situ hybridization for the detection of nitrifying bacteria in activated sludge and biofilms, and to determine the distribution of nitrifiers in selected wastewater treatment plants (WWTPs). Both Czech and foreign WWTPs with intensification of nitrification (for example, in situ bioaugmentation of nitrification or biofilms) and without intensification were studied. The two-dimensional and three-dimensional analyses of microscopic images were focused on quantifying the parameters and their differences with regard to the arrangement, capacity and sludge age of the WWTPs. This is the first time such a study has been performed in the Czech Republic.

中文翻译：

---

荧光原位杂交技术在硝化/反硝化污水处理厂硝化细菌研究和表征中的应用

本研究的目的是验证荧光原位杂交用于检测活性污泥和生物膜中的硝化细菌，并确定选定污水处理厂 (WWTP) 中硝化菌的分布。捷克和外国污水处理厂都进行了强化硝化（例如，硝化或生物膜的原位生物强化）和没有强化的污水处理厂。微观图像的二维和三维分析侧重于量化参数及其在污水处理厂的布置、容量和污泥龄方面的差异。这是捷克共和国首次进行此类研究。