B7-H3 and B7-H4 play an inhibitory role in T-cell function by limiting proliferation and cytokine production. Information about B7-H3 and B7-H4 expression in ductal carcinoma in situ (DCIS) remains uncertain. The objective of this study was to evaluate the expression levels of B7-H3 and B7-H4 in DCIS and their associations with clinicopathologic features and T-cell infiltration. B7-H3 and B7-H4 mRNA and protein expression levels in 8 pairs of DCIS tissues and matched normal adjacent tissues were examined by RNAscope in situ hybridization and immunohistochemistry analysis. Immunohistochemical staining of B7-H3, B7-H4, CD3, and CD8 was performed for 79 DCIS samples using tissue microarray. RNAscope in situ hybridization and immunohistochemistry analysis revealed that expression levels of B7-H3 and B7-H4 in DCIS tissues were higher than those in corresponding normal tissues. B7-H3 and B7-H4 mRNA and protein appeared to be mainly expressed in DCIS carcinoma cells. High B7-H3 and B7-H4 expression was observed in 58 (73.4%) and 62 (78.5%) cases with DCIS, respectively. High B7-H3 expression was significantly associated with high-nuclear grade and presence of comedo-type necrosis (both P<0.05). B7-H3 expression in HR−/HER2+ subtype was higher than that in HR+/HER2− subtype (P<0.05). B7-H3 and B7-H4 expression levels were negatively related to the density of CD3+ and CD8+ T-cell infiltrates. B7-H3 and B7-H4 may play an important role in immune surveillance mechanisms of DCIS. They might be useful targets to develop immune-based therapy to alter or prevent DCIS progression.

中文翻译：

---

乳腺导管原位癌中 B7-H3 和 B7-H4 表达与临床病理特征和 T 细胞浸润的关联

B7-H3 和 B7-H4 通过限制增殖和细胞因子的产生在 T 细胞功能中发挥抑制作用。关于 B7-H3 和 B7-H4 在导管原位癌 (DCIS) 中表达的信息仍不确定。本研究的目的是评估 DCIS 中 B7-H3 和 B7-H4 的表达水平及其与临床病理特征和 T 细胞浸润的关联。采用RNAscope原位杂交和免疫组化分析法检测8对DCIS组织及匹配的正常邻近组织中B7-H3和B7-H4的mRNA和蛋白表达水平。使用组织微阵列对 79 个 DCIS 样品进行 B7-H3、B7-H4、CD3 和 CD8 的免疫组织化学染色。RNAscope原位杂交和免疫组化分析显示DCIS组织中B7-H3和B7-H4的表达水平高于相应的正常组织。B7-H3 和 B7-H4 mRNA 和蛋白质似乎主要在 DCIS 癌细胞中表达。分别在 58 (73.4%) 和 62 (78.5%) 例 DCIS 中观察到高 B7-H3 和 B7-H4 表达。高 B7-H3 表达与高核分级和粉刺型坏死的存在显着相关（均 P<0.05）。B7-H3在HR-/HER2+亚型中的表达高于HR+/HER2-亚型（P<0.05）。B7-H3 和 B7-H4 表达水平与 CD3+ 和 CD8+ T 细胞浸润的密度呈负相关。B7-H3 和 B7-H4 可能在 DCIS 的免疫监视机制中起重要作用。