Flow cytometry is a versatile and high throughput technique for rapid and efficient biological testing. It requires a high level of conceptual, technical, and analytical skills to properly design experiments, effectively operate flow cytometry machines, and analyze the data. A lack of training and development of any of these three skills can result in underutilization and improper use of flow cytometric machines that can impede research progress. Often students develop these conceptual, technical, and analysis skills from trial and error, but many students either do not use this powerful flow cytometry technology, use it improperly or ineffectively, or give up using it without proper training and support. Here we report on a course which teaches flow cytometry skills to undergraduate and graduate students. The design of this course is unique in that it teaches conceptual, technical, and analytical skills related to flow cytometry in a full semester format. Undergraduate and graduate students reported significant increases in their confidence levels over the course of the semester. Here we provide our findings and resources for others who may want to implement a similar course.

中文翻译：

---

一门完整的流式细胞术课程提高了研究生和本科生的信心

流式细胞术是一种通用的高通量技术，可用于快速高效的生物检测。它需要高水平的概念、技术和分析技能来正确设计实验、有效操作流式细胞仪和分析数据。缺乏这三项技能中的任何一项的培训和发展都可能导致流式细胞仪的未充分利用和不当使用，从而阻碍研究进展。学生通常通过反复试验来培养这些概念、技术和分析技能，但许多学生要么不使用这种强大的流式细胞术技术，要么使用不当或无效，要么在没有适当培训和支持的情况下放弃使用它。在这里，我们报告了一门向本科生和研究生教授流式细胞术技能的课程。本课程的设计独一无二，因为它以一学期的形式教授与流式细胞术相关的概念、技术和分析技能。本科生和研究生报告说，在整个学期中，他们的信心水平显着提高。在这里，我们为可能想要实施类似课程的其他人提供我们的发现和资源。