Sequencing of the black rockfish chromosomal genome provides insight into sperm storage in the female ovary.,DNA Research

当前位置： X-MOL 学术 › DNA Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Sequencing of the black rockfish chromosomal genome provides insight into sperm storage in the female ovary.
DNA Research ( IF 3.9 ) Pub Date : 2019-12-01 , DOI: 10.1093/dnares/dsz023
Qinghua Liu _{1,

2,

3} , Xueying Wang _{1,

2,

3} , Yongshuang Xiao _{1,

2,

3} , Haixia Zhao _{1,

2,

3,

4} , Shihong Xu _{1,

2,

3} , Yanfeng Wang _{1,

2,

3} , Lele Wu _{1,

2,

3,

4} , Li Zhou _{1,

2,

3,

4} , Tengfei Du _{1,

2,

3,

4} , Xuejiao Lv _{1,

2,

3,

4} , Jun Li _{1,

2,

3}

Affiliation

Black rockfish (Sebastes schlegelii) is an economically important viviparous marine teleost in Japan, Korea, and China. It is characterized by internal fertilization, long-term sperm storage in the female ovary, and a high abortion rate. For better understanding the mechanism of fertilization and gestation, it is essential to establish a reference genome for viviparous teleosts. Herein, we used a combination of Pacific Biosciences sequel, Illumina sequencing platforms, 10× Genomics, and Hi-C technology to obtain a genome assembly size of 848.31 Mb comprising 24 chromosomes, and contig and scaffold N50 lengths of 2.96 and 35.63 Mb, respectively. We predicted 39.98% repetitive elements, and 26,979 protein-coding genes. S. schlegelii diverged from Gasterosteus aculeatus ∼32.1-56.8 million years ago. Furthermore, sperm remained viable within the ovary for up to 6 months. The glucose transporter SLC2 showed significantly positive genomic selection, and carbohydrate metabolism-related KEGG pathways were significantly up-regulated in ovaries after copulation. In vitro suppression of glycolysis with sodium iodoacetate reduced sperm longevity significantly. The results indicated the importance of carbohydrates in maintaining sperm survivability. Decoding the S. schlegelii genome not only provides new insights into sperm storage; additionally, it is highly valuable for marine researchers and reproduction biologists.

更新日期：2019-11-01

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11