Nowadays, cancer disease is continuously identified as the leading cause of mortality worldwide. Cancer chemotherapeutic agents have been continuously developing to achieve high curative effectiveness and low side effects. However, solid tumors present the properties of low drug penetration and resistance of quiescent cells. Radiation therapy is concurrently given in some cases; but it induces different levels of adverse effects. In the current work, uniform sized multicellular spheroids were raised by microwell arrays to mimic the architecture of solid tumors. Investigation of the response of the spheroids was conducted after the treatment of alternating electric field. The result showed that the electric field could induce early apoptosis by disturbing cell membrane. Moreover, combined treatment of electric field and anti-cancer drug was applied to the spheroids. The electric field synergistically enhanced the treatment efficacy because the anti-cancer drug could permeate through the disrupted cell membrane. Significant improvement of late apoptosis was shown by the combined treatment. Because the electric field treatment induces limited side effect to the patient, lower dosage of anti-cancer drug may be applied to the patients for achieving curative effectiveness.

中文翻译：

---

电场和抗癌药联合处理后，微孔阵列产生的均一大小的多细胞球体的研究。

如今，癌症一直被认为是世界范围内导致死亡的主要原因。癌症化学治疗剂一直在不断发展，以实现高疗效和低副作用。然而，实体瘤表现出低药物渗透性和静态细胞抗性的特性。在某些情况下，同时进行放射治疗。但会引起不同程度的不良影响。在当前的工作中，通过微孔阵列培养出大小一致的多细胞球体，以模仿实体瘤的结构。在交变电场处理之后，对球体的响应进行了研究。结果表明，电场可通过干扰细胞膜诱导早期凋亡。此外，电场和抗癌药的联合治疗应用于球体。电场协同增强了治疗效果，因为抗癌药可能会穿透破裂的细胞膜。联合治疗显示晚期细胞凋亡显着改善。因为电场治疗对患者产生有限的副作用，所以可以将较低剂量的抗癌药应用于患者以获得疗效。