As we all know, microvalve holds great importance for microfluidic manipulation in chip. Herein, a simple method of high-performance multiplex microvalves chip fabrication was reported. In this method, a sandwich structure is established by inserting a polydimethylsiloxane (PDMS) membrane into two glasses, which is cheap and simple without any complex silicon-based device or soft lithography. Taking advantages of both the elasticity of the PDMS and the rigidity of glass, the microvalve chip showed good controls performance and had the ability of multiplex integration. Moreover, aided by a computer design program, this microvalves chip can be performed automatically, showing great potential to develop new highly integrated microfluidic devices. In addition, the fabricated multiplex microvalve chip is further successfully used for staining tumor cells automatically, improving the efficiency of cell identification process and reducing human errors. These results indicate this method opens up new avenues for multiplex microvalves fabrication and its biological application.

中文翻译：

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高性能多重微阀的制造，并用于微流控芯片上的肿瘤细胞染色。

众所周知，微阀对于芯片中的微流体操作非常重要。在此，报道了一种高性能的多重微阀芯片制造的简单方法。在这种方法中，通过将聚二甲基硅氧烷（PDMS）膜插入两块玻璃中来建立三明治结构，这种结构便宜且简单，无需任何复杂的基于硅的器件或软光刻。该微阀芯片兼具PDMS的弹性和玻璃的刚性，显示出良好的控制性能，并具有多重集成的能力。而且，借助计算机设计程序，该微阀芯片可以自动执行，显示出开发新的高度集成的微流控设备的巨大潜力。此外，所制备的多重微阀芯片进一步成功地用于肿瘤细胞的自动染色，提高了细胞鉴定过程的效率，减少了人为错误。这些结果表明该方法为多重微阀的制备及其生物学应用开辟了新的途径。