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Super resolution NOESY spectra of proteins.
Journal of Biomolecular NMR ( IF 2.4 ) Pub Date : 2019-05-02 , DOI: 10.1007/s10858-019-00231-x
Charles F DeLisle 1 , H Bhagya Mendis 1 , Justin L Lorieau 1
Affiliation  

Spectral resolution remains one of the most significant limitations in the NMR study of biomolecules. We present the srNOESY (super resolution nuclear overhauser effect spectroscopy) experiment, which enhances the resolution of NOESY cross-peaks at the expense of the diagonal peak line-width. We studied two proteins, ubiquitin and the influenza hemagglutinin fusion peptide in bicelles, and we achieved average resolution enhancements of 21-47% and individual peak enhancements as large as ca. 450%. New peaks were observed over the conventional NOESY experiment in both proteins as a result of these improvements, and the final structures generated from the calculated restraints matched published models. We discuss the impact of the experimental parameters, spin diffusion and the information content of the srNOESY lineshape.

中文翻译:

蛋白质的超高分辨率NOESY光谱。

光谱分辨率仍然是生物分子NMR研究中最重要的限制之一。我们提出了srNOESY(超分辨率核Overhauser效应光谱)实验,该实验以对角峰线宽为代价提高了NOESY交叉峰的分辨率。我们在单细胞中研究了两种蛋白质,泛素和流感血凝素融合肽,我们实现了21-47%的平均分离度增强和最大约为ca的单个峰增强。450%。由于这些改进,两种蛋白质在常规NOESY实验中均观察到了新的峰,并且从计算出的约束条件生成的最终结构与已发表的模型相匹配。我们讨论了实验参数,自旋扩散和srNOESY线形的信息内容的影响。
更新日期:2019-04-30
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