Glycogen is a primary metabolic reserve in bivalves and can be suitable for the evaluation of bivalve condition and health status, but the use of glycogen as a diagnostic tool in aquaculture and biomonitoring is still relatively rare. A tissue biopsy combined with a simplified phenol-sulfuric acid method was used in this study to evaluate the inter- and intraindividual variation in the glycogen concentrations among several tissues (foot, mantle, gills, adductor muscle) of the unionid bivalve, the duck mussel Anodonta anatina. This short report documents that individual bivalves differ in the spatial distribution of glycogen among tissues. Sampling of different types of tissues can cause distinct results in the evaluation of energetic reserves at the individual level. At the same time, spatial variability in glycogen content has the potential to provide a more detailed evaluation of physiological conditions based on tissue-specific glycogen storage. The results obtained and the simplified methodology provide a new opportunity for researching the energetic reserves and health status of freshwater mussels in various applications.

中文翻译：

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淡水双壳类组织糖原分布的变化：简化方案和意义

糖原是双壳类动物的主要代谢储备，可适用于评估双壳类动物的状况和健康状况，但将糖原用作水产养殖和生物监测的诊断工具还比较少见。本研究使用组织活检结合简化的苯酚 - 硫酸法来评估双壳贝类、鸭贻贝的多个组织（足、地幔、鳃、内收肌）中糖原浓度的个体间和个体间变化Anodonta anatina。这份简短的报告记录了单个双壳类动物在组织间糖原空间分布方面的差异。对不同类型组织的取样可以在个体水平的能量储备评估中产生不同的结果。同时，糖原含量的空间变异性有可能根据组织特异性糖原储存对生理条件进行更详细的评估。获得的结果和简化的方法为研究淡水贻贝在各种应用中的能量储备和健康状况提供了新的机会。