The present study was conducted to explore the occurrence of Flavobacteriaceae in wild Nile Tilapia Oreochromis niloticus (n = 108) collected from Lake Victoria and farmed Nile Tilapia (n = 187) collected from 12 ponds in the Morogoro region of Tanzania. The size of the ponds surveyed ranged from 130 to 150 m2 . Pond parameters and fish morphometric data were recorded during sampling. In total, 67 Flavobacterium-like isolates (n = 44 from farmed fish; n = 23 from wild fish) were identified on the basis of colony morphology and biochemical tests. Sequences from the 16S ribosomal RNA (rRNA) gene revealed that all 67 isolates belonged to the genera Flavobacterium and Chryseobacterium. Based on 16S rRNA nucleotide identity, 26 isolates showed high similarity with C. indologenes (99-100% identity), 16 showed similarity to C. joostei (98-99.9%), and 17 were similar to diverse species of Chryseobacterium (97-99%). Three isolates were similar to F. aquatile and three were similar to F. indicum, with 99-100% nucleotide identity in both cases, and two isolates were similar to F. oryzae (99-100% identity). The findings obtained in this study provide a baseline for future studies and contribute to an understanding of the threats presented by the aquatic Flavobacteriaceae reservoir toward the development of healthy fish farming in Tanzania. Such knowledge is vital for the development of a sustainable aquaculture industry in Tanzania that will contribute to increased food security.

中文翻译：

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坦桑尼亚养殖和野生尼罗罗非鱼黄杆菌科的分离与鉴定

本研究旨在探讨从维多利亚湖采集的野生尼罗罗非鱼 Oreochromis niloticus (n = 108) 和从坦桑尼亚莫罗戈罗地区 12 个池塘采集的养殖尼罗罗非鱼 (n = 187) 中黄杆菌科的发生情况。所调查的池塘面积介于 130 至 150 平方米之间。在取样期间记录池塘参数和鱼类形态测量数据。根据菌落形态和生化测试，总共鉴定出 67 个类黄杆菌分离株（n = 44 来自养殖鱼类；n = 23 来自野生鱼类）。来自 16S 核糖体 RNA (rRNA) 基因的序列显示，所有 67 个分离株都属于黄杆菌属和金黄色杆菌属。基于 16S rRNA 核苷酸同一性，26 个分离株与 C. indologenes 表现出高度相似性（99-100% 同一性），16 个与 C. joostei（98-99.9%）表现出相似性，和 17 种与不同种类的金黄杆菌相似（97-99%）。三个分离物与 F. aquatile 相似，三个与 F. indicum 相似，在两种情况下都具有 99-100% 的核苷酸同一性，并且两个分离物与 F. oryzae 相似（99-100% 同一性）。本研究中获得的发现为未来的研究提供了基线，并有助于了解水生黄杆菌科水库对坦桑尼亚健康养鱼业发展的威胁。这些知识对于坦桑尼亚可持续水产养殖业的发展至关重要，这将有助于提高粮食安全。和两个分离株与稻瘟病菌相似（99-100% 同一性）。本研究中获得的发现为未来的研究提供了基线，并有助于了解水生黄杆菌科水库对坦桑尼亚健康养鱼业发展的威胁。这些知识对于坦桑尼亚可持续水产养殖业的发展至关重要，这将有助于提高粮食安全。和两个分离株与稻瘟病菌相似（99-100% 同一性）。本研究中获得的发现为未来的研究提供了基线，并有助于了解水生黄杆菌科水库对坦桑尼亚健康养鱼业发展的威胁。这些知识对于坦桑尼亚可持续水产养殖业的发展至关重要，这将有助于提高粮食安全。