Attached growth reactors were developed separately for solids retention time (SRT)-controlled partial nitrification and for anaerobic ammonia oxidation (Anammox) treatment, and a new nitrogen removal process is proposed for wastewater containing highly concentrated ammonia. For partial nitrification, an attached growth medium of polyurethane foam was used. Partial nitrification was achieved stably under a SRT of 4 days, and the abundance ratio of NO -N to the sum of NH -N, NO -N and NO -N was approximately 0.8 after 10 days. Under a SRT of 4 days, the amoA gene concentrations of ammonia-oxidizing bacteria increased from 1×108 to 7×108 copies/l, whereas the 16S ribosomal RNA (16S rRNA) gene concentrations of nitrite-oxidizing bacteria did not increase. These results indicate that SRT-controlled operation is a promising technology for achieving partial nitrification. For the Anammox treatment, an attached growth medium of non-woven fabric was used. Inorganic nitrogen removal of approximately 80–90% was observed at an inorganic nitrogen loading rate of over 10 kgN/(m3-medium·d) and an influent nitrogen concentration of 400 mgN/l. Our non-woven fabric reactor showed similar or superior Anammox performance to that reported previously. By using a combination of these two rectors, we can develop a method that combines partial nitrification and Anammox treatment for effective and stable nitrogen removal.

中文翻译：

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部分硝化与厌氧氨氧化联合处理去除废水中高浓度氨

针对固体保留时间 (SRT) 控制的部分硝化和厌氧氨氧化 (Anammox) 处理分别开发了附加生长反应器，并针对含有高浓度氨的废水提出了一种新的脱氮工艺。对于部分硝化，使用附着的聚氨酯泡沫生长培养基。部分硝化在4天的SRT下稳定实现，10天后NO -N与NH -N、NO -N和NO -N总和的丰度比约为0.8。在 4 天的 SRT 下，氨氧化细菌的 amoA 基因浓度从 1×108 增加到 7×108 拷贝/升，而亚硝酸盐氧化细菌的 16S 核糖体 RNA（16S rRNA）基因浓度没有增加。这些结果表明 SRT 控制操作是实现部分硝化的有前途的技术。对于厌氧氨氧化处理，使用无纺布的附着生长培养基。在无机氮负载率超过 10 kgN/(m3-medium·d) 和进水氮浓度为 400 mgN/l 时，观察到无机氮去除率约为 80-90%。我们的无纺布反应器显示出与之前报道的相似或优越的 Anammox 性能。通过结合使用这两个反应器，我们可以开发一种结合部分硝化和厌氧氨氧化处理的方法，以实现有效和稳定的脱氮。在无机氮负载率超过 10 kgN/(m3-medium·d) 和进水氮浓度为 400 mgN/l 时，观察到无机氮去除率约为 80-90%。我们的无纺布反应器显示出与之前报道的相似或优越的 Anammox 性能。通过结合使用这两个反应器，我们可以开发一种结合部分硝化和厌氧氨氧化处理的方法，以实现有效和稳定的脱氮。在无机氮负载率超过 10 kgN/(m3-medium·d) 和进水氮浓度为 400 mgN/l 时，观察到无机氮去除率约为 80-90%。我们的无纺布反应器显示出与之前报道的相似或优越的 Anammox 性能。通过结合使用这两个反应器，我们可以开发一种结合部分硝化和厌氧氨氧化处理的方法，以实现有效和稳定的脱氮。