The wide range of potential health beneficial effects of isoflavones, including a chemoprentive action, have prompted us to study the potential benefits of genistein and daidzein in an experimental model of environmental pollution impact on lung tissue. A diesel engine placed was used to generate reproducible emissions including both gaseous and particulate matters that are commonly found in urban atmospheres. Isoflavones were added to culture medium of rat lung slices 2 h prior to their exposure to pollutants for 3 h. Intracellular ATP and GSH levels, TNFα production, nucleosome assay and TUNEL labeling were monitored. Isoflavones showed almost total in vitro protection against inflammatory and pro-apoptotic responses in lung slices. Isoflavones 0.3 and 1 μmol/l protected against exhaust induced GSH depletion. Isoflavones 0.3 μmol/l appeared to exert the most beneficial effects. In conclusion, this study points out the potential interest of soy isoflavones consumption in polluted areas. Further studies should be undertaken to verify that similar effects could be obtained after in vivo administration of isoflavones.

中文翻译：

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异黄酮可防止肺组织器官培养中的柴油机排气损伤。

异黄酮具有广泛的潜在健康益处，包括化学作用，促使我们在环境污染对肺组织的影响的实验模型中研究染料木黄酮和大豆苷元的潜在益处。放置的柴油发动机用于产生可再现的排放物，包括城市大气中常见的气态和颗粒物。在将异黄酮暴露于污染物3小时之前，将其添加到大鼠肺切片的培养基中2小时。监测细胞内ATP和GSH水平，TNFα产生，核小体测定和TUNEL标记。异黄酮对肺片中的炎症和促凋亡反应具有几乎完全的体外保护作用。0.3和1μmol/ l的异黄酮可防止排气引起的GSH消耗。异黄酮0。3μmol/ l似乎发挥了最有益的作用。总之，本研究指出了在污染地区食用大豆异黄酮的潜在利益。应当进行进一步的研究以证实在体内施用异黄酮后可以获得类似的效果。