A new radiation biotechnology for the acquirement of a commercial vaccine, designed for prophylaxis of ruminant infectious pododermatitis (IP), produced by gram negative bacteria Fuso-bacterium necrophorum (F. n.), is presented. Two different processes for preparing F. n. vaccine are used: a) the inactivation of F. n. bacteria exotoxins by microwave (MW) or/and electron beams (EB) irradiation; b) the isolation of exotoxins from F. n. cultures irradiated with MW or/and EB and the inactivation of isolated F. n. exotoxins with formalin. The EB irradiation of F. n. cultures produced simultaneously with the cells viability decrease an increasing of exotoxin quantity released in the culture supranatant as compared with classical methods. The MW irradiation is able to reduce the cells viability to zero but without an increase of exotoxin quantity in cultures supranatant. Instead of this MW irradiation, for certain conditions, is able to induce an important stimulation degree of the F. n. proliferation in cultures, from two to three log10. Two vaccine types were prepared: A1 vaccine that contains whole cell culture irradiated with MW/EB and A2 vaccine that contains cell-free culture supernatant of an MW/EB irradiated F. n. strain producing exotoxins. Also, other two vaccines are prepared: B1 and B2 that contain the same materials as A1 and A2 respectively, but without using MW/EB exposure. The vaccine efficiency is tested in ruminant farms in which IP evolves. It is expected that this new vaccine to offer a better protection, more than 60%, which is the best presently obtained result in ruminant farms.

中文翻译：

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通过辐射处理制备疫苗

介绍了一种用于获得商业疫苗的新辐射生物技术，该疫苗设计用于预防由革兰氏阴性菌 Fuso-bacterium necrophorum (F. n.) 产生的反刍动物传染性足皮炎 (IP)。制备 F. n. 的两种不同工艺。使用疫苗： a) F. n. 的灭活。通过微波 (MW) 或/和电子束 (EB) 辐射的细菌外毒素；b) 从 F. n. 中分离外毒素。用 MW 或/和 EB 照射的培养物和分离的 F. n. 的灭活。外毒素与福尔马林。F. n. 的 EB 辐照。与传统方法相比，与细胞活力同时产生的培养物减少了培养物上清液中释放的外毒素量的增加。MW 辐射能够将细胞活力降低至零，但不会增加上层培养物中的外毒素量。代替这种 MW 照射，在某些条件下，能够诱导 F.n 的重要刺激程度。文化中的增殖，从两个到三个 log10。制备了两种疫苗类型：包含用 MW/EB 辐照的全细胞培养物的 A1 疫苗和包含经 MW/EB 辐照的 F.n. 的无细胞培养上清液的 A2 疫苗。产生外毒素的菌株。此外，还制备了另外两种疫苗：B1 和 B2，它们分别含有与 A1 和 A2 相同的材料，但不使用 MW/EB 暴露。在 IP 进化的反刍动物农场中测试疫苗效率。预计这种新疫苗能提供更好的保护，超过 60%，