Ghrelin and leptin regulate appetite and energy homeostasis in humans and rodents. The effects of different nutritional factors on ghrelin and leptin secretion are not well documented in rats. Therefore, the aim of our study was to investigate the effect of a high-fat diet on plasma ghrelin and leptin levels and on adiposity. Twenty male Wistar rats, body weight220–260 g, were used in the study. Rats were randomized either on a standard chow diet (n=10) or on a high-fat diet (a mixture of nuts) forad libitum 11-week period. Body weight was measured once per week. At the end of the nutritional period, rats were sacrificed. Blood was collected for determination of lipids and glucose, as well as plasma ghrelin and leptin levels by ELISA method. The weight of different organs was determined. Rats fed on a high-fat diet showed significant increase in total body weight compared to control group. The long-term intake of high-fat diet caused hyperleptinemia and hypoghrelinemia. There was a significant positive correlation between plasma leptin levels and epididymal fat mass, liver and heart. In contrast, ghrelin levels showed inverse correlation with epididymal fat mass and liver weight. In conclusion, long-term intake of high-fat diet induced changes in plasma ghrelin and leptin in male rats, as well as in epididymal fat mass, liver and heart weights.

中文翻译：

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高脂饮食对大鼠血浆生长素释放肽和瘦素水平的影响。

胃饥饿素和瘦素调节人类和啮齿动物的食欲和能量稳态。不同营养因素对大鼠胃饥饿素和瘦素分泌的影响尚未得到充分证明。因此，我们研究的目的是研究高脂肪饮食对血浆生长素释放肽和瘦素水平以及肥胖的影响。研究中使用了 20 只雄性 Wistar 大鼠，体重为220-260克。大鼠随机接受标准食物（n = 10）或高脂肪饮食（坚果混合物）随意11 周期间。每周测量一次体重。在营养期结束时，处死大鼠。收集血液以通过ELISA方法测定脂质和葡萄糖，以及血浆生长素释放肽和瘦素水平。测定不同器官的重量。与对照组相比，以高脂肪饮食喂养的大鼠的总体重显着增加。长期摄入高脂肪饮食引起高瘦素血症和低饥饿素血症。血浆瘦素水平与附睾脂肪量、肝脏和心脏呈显着正相关。相反，生长素释放肽水平与附睾脂肪量和肝脏重量呈负相关。总之，长期摄入高脂饮食会导致雄性大鼠血浆生长素释放肽和瘦素的变化，以及附睾脂肪量、肝脏和心脏重量的变化。