Thrombotic diseases or fatalities have been reported to occasionally occur under conditions of hypergravity although the mechanism is still unclear. To investigate the effect of hypergravity on platelets that are the primary players in thrombus formation, platelet rich plasma (PRP) or washed platelets were exposed to hypergravity at 8 G for 15 minutes. No platelet aggregation was induced by 8 G alone, whereas ristocetin or collagen-induced platelet aggregation was significantly increased. The number of platelets adherent to immobilized fibrinogen and the area of platelets spreading on von Willbrand factor (VWF) matrix were increased simultaneously. Flow cytometry assay indicated that integrin αIIbß3 was partially activated in 8 Gexposed platelets, but there was no significant difference in P-selectin surface expression between platelets treated with 8 G and 1 G control. The results indicate that hypergravity leads to human platelet hyperactivity, but fails to incur essential platelet activation events, suggesting a novel mechanism for thrombotic diseases occurring under hypergravitional conditions.

中文翻译：

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超重力导致人类血小板过度活跃。

据报道，在超重力条件下偶尔会发生血栓性疾病或死亡，尽管其机制尚不清楚。为了研究超重力对作为血栓形成主要参与者的血小板的影响，富血小板血浆 (PRP) 或洗涤的血小板在 8 G 的超重力下暴露 15 分钟。单独使用 8 G 没有诱导血小板聚集，而瑞斯托霉素或胶原诱导的血小板聚集显着增加。粘附在固定纤维蛋白原上的血小板数量和血小板在血管性血友病因子 (VWF) 基质上的扩散面积同时增加。流式细胞术分析表明整合素 αIIbß3 在 8 个 Gexposed 血小板中被部分激活，但在用 8 G 和 1 G 对照处理的血小板之间 P-选择素表面表达没有显着差异。结果表明，超重力导致人类血小板过度活跃，但不会引起基本的血小板活化事件，这表明在超重力条件下发生血栓性疾病的新机制。