Abstract Surface pre-reacted glass-ionomer (S-PRG) is a bioactive filler produced by PRG technology, which is applied to various dental materials. The inhibitory effects of S-PRG eluate against Candida, the most common fungal oral pathogen, were investigated. Minimum inhibitory concentrations (MIC) and anti-biofilm activities were tested against Candida albicans, Candida glabrata, Candida krusei, and Candida tropicalis. For the in vivo study, Galleria mellonella was used as a model to evaluate the effects of S-PRG on toxicity, hemocyte counts and candidiasis. The MIC of S-PRG ranged from 5 to 40% (v/v). S-PRG eluate exhibited anti-biofilm activity for all the Candida species tested. Furthermore, injection of S-PRG eluate into G. mellonella was not toxic to the larvae and protected G. mellonella against experimental candidiasis. In addition, S-PRG eluate inhibited biofilm formation by C. albicans, C. glabrata, C. krusei, and C. tropicalis and exerted protective effects on G. mellonella against experimental candidiasis in vivo.

中文翻译：

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表面预反应玻璃离子 (S-PRG) 洗脱液对念珠菌的影响：抗真菌活性、抗生物膜特性以及​​对大蜡螟对白色念珠菌感染的保护作用

摘要 表面预反应玻璃离子（S-PRG）是一种利用PRG技术生产的生物活性填料，应用于各种牙科材料。研究了 S-PRG 洗脱液对念珠菌的抑制作用，念珠菌是最常见的口腔真菌病原体。针对白色念珠菌、光滑念珠菌、克柔念珠菌和热带念珠菌测试了最小抑制浓度 (MIC) 和抗生物膜活性。在体内研究中，大蜡螟被用作评估 S-PRG 对毒性、血细胞计数和念珠菌病影响的模型。S-PRG 的 MIC 范围为 5% 至 40% (v/v)。S-PRG 洗脱液对所有测试的念珠菌物种都表现出抗生物膜活性。此外，将 S-PRG 洗脱液注射到 G. mellonella 中对幼虫没有毒性，并保护 G. mellonella 免受实验性念珠菌病的侵害。此外，