A whole-genome resequencing-derived SNP dataset identified six quantitative trait loci (QTL) significantly associated with colonization of soybean by an arbuscular mycorrhizal fungus (Rhizophagus intraradices). Candidate genes identified in these QTL regions include homologs to known nodulin protein families and other symbiosis-specific genes. Arbuscular mycorrhizal fungi (AMF) form associations with over 80% of all terrestrial plant species and assist their host plants by increasing their nutrient uptake, drought tolerance, and resilience against pathogens and pests. Genotypic variation of crop plants to AMF colonization has been identified in crops, including soybean; however, the genetics controlling levels of AMF colonization in soybean are unknown. The overall goal of our study was to identify genomic regions associated with mycorrhizal colonization in soybean using genome-wide association analysis. A diverse panel of 350 exotic soybean genotypes inoculated with Rhizophagus intraradices were microscopically evaluated for root colonization using a modified gridline intersect method. Root colonization differed significantly (P < 0.001) among genotypes and ranged from 11 to 70%. A whole-genome resequencing-derived SNP dataset identified six quantitative trait loci (QTL) significantly associated with R. intraradices colonization that explained 24% of the phenotypic variance. Candidate genes identified in these QTL regions include homologs to known nodulin protein families and other symbiosis-specific genes. The results showed there was a significant genetic component to the level of colonization by R. intraradices in soybean. This information may be useful in the development of AMF-sensitive soybean cultivars to enhance nutrient uptake, drought tolerance, and disease resistance in the crop.

中文翻译：

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全基因组重测序鉴定了与大豆菌根定植相关的数量性状位点。


全基因组重测序衍生的 SNP 数据集确定了 6 个数量性状位点 (QTL)，这些位点与丛枝菌根真菌（根内根噬菌）对大豆的定植显着相关。在这些 QTL 区域中鉴定的候选基因包括已知结节蛋白家族和其他共生特异性基因的同源物。丛枝菌根真菌 (AMF) 与 80% 以上的陆地植物物种形成关联，并通过增加宿主植物的养分吸收、耐旱性以及对病原体和害虫的抵抗力来帮助宿主植物。已经在包括大豆在内的农作物中发现了 AMF 定植的基因型变异；然而，控制大豆中 AMF 定植水平的遗传学尚不清楚。我们研究的总体目标是利用全基因组关联分析来识别与大豆中菌根定植相关的基因组区域。使用改良的网格线相交方法，对接种了根内根噬菌的 350 种外来大豆基因型的不同组进行了显微镜下的根部定殖评估。不同基因型的根部定殖差异显着（P < 0.001），范围为 11% 至 70%。全基因组重测序衍生的 SNP 数据集确定了与根内根瘤菌定植显着相关的 6 个数量性状位点 (QTL)，这解释了 24% 的表型变异。在这些 QTL 区域中鉴定的候选基因包括已知结节蛋白家族和其他共生特异性基因的同源物。结果表明，根内根瘤菌在大豆中的定殖水平存在显着的遗传因素。 这些信息可能有助于开发对 AMF 敏感的大豆品种，以增强作物的养分吸收、耐旱性和抗病性。