BACKGROUND Various tissue culture medium formulations currently are used to transport, store and process stem and progenitor cells for transplantation, although these solutions are not manufactured for clinical use. METHODS We have developed an infusible-grade solution, STM-Sav, for short-term liquid cell storage and tested its ability to maintain viable functional hematopoietic cells, compared with commonly-used tissue culture solutions. G-CSF-stimulated normal-donor PBPC were stored in alpha-MEM, IMDM, RPMI-1640, AIM 5, X-VIVO 10, PlasmaLyte A and STM-Sav, in gas-permeable Cryocyte bags, at ambient temperature and 4 degrees C. The percentage of viable cells, percent recovery of viable mononuclear cells (MNC), percentage of CD34+ cells, CFU-GM frequency and solution pH were determined for each solution, at time points ranging from 0 to 72 h. RESULTS Cells were slightly better maintained at 4 degrees C than at ambient temperature. No solution was superior to any other overall, although PlasmaLyte A was significantly inferior to all other solutions at preserving viable MNC. STM-Sav consistently maintained viable, functional cells as well as, or better than in-vitro-use-only culture media, or PlasmaLyte A. DISCUSSION Cells could be stored in STM-Sav for at least 24 h at 4 degrees C or ambient temperature, with recovery of approximately 90% of initial viable MNC, unchanged percentage CD34+ cells and stable solution pH.

中文翻译：

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用于非冷冻保存的造血细胞储存的不溶级解决方案的开发

背景技术目前使用各种组织培养基制剂来运输、储存和加工用于移植的干细胞和祖细胞，尽管这些溶液不是为临床使用而制造的。方法 我们开发了一种用于短期液体细胞储存的难熔级溶液 STM-Sav，并测试了与常用的组织培养溶液相比，其维持活的功能性造血细胞的能力。G-CSF 刺激的正常供体 PBPC 储存在 alpha-MEM、IMDM、RPMI-1640、AIM 5、X-VIVO 10、PlasmaLyte A 和 STM-Sav 中，在透气的冷冻细胞袋中，室温和 4 度C. 确定每个溶液的活细胞百分比、活单核细胞 (MNC) 回收百分比、CD34+ 细胞百分比、CFU-GM 频率和溶液 pH 值，时间点范围为 0 到 72 小时。结果 与环境温度相比，细胞在 4 摄氏度下的保存情况稍好一些。尽管 PlasmaLyte A 在保留可行的 MNC 方面明显低于所有其他解决方案，但没有任何解决方案优于其他任何解决方案。STM-Sav 始终保持活的、有功能的细胞以及或优于仅在体外使用的培养基或 PlasmaLyte A。讨论 细胞可以在 STM-Sav 中在 4 摄氏度或环境温度下储存至少 24 小时温度，恢复约 90% 的初始存活 MNC，CD34+ 细胞百分比不变，溶液 pH 值稳定。