BACKGROUND G-CSF administration over 10 days and neutrophil cytapheresis have been reported in the literature, but the kinetics of CD34+ cells in this situation is unclear. CASE A 42-year-old female underwent syngeneic transplantation for metastatic breast cancer. The recipient was in critical condition peri-transplant, therefore the donor received G-CSF for 13 days, during which eight cytaphereses for both PBPC and neutrophils were performed. Two peaks in CD34+ cells were noted; the first on Day 5 and the second on Days 10-13 of G-CSF administration; a total of 11.6 x 10(6)/kg CD34+ cells and 38.11 x 10(8)/kg neutrophils were infused. The recipient's ANC exceeded 0.1 x 10(9)/L on Day +3. DISCUSSION To our knowledge, this is the longest reported cytapheresis of CD34+ cells from a normal donor The bi-phasic pattern in the cytapheresis product is also of interest. It is an unusual pattern that suggests a profound and complicated alteration in the marrow progenitor cell pool. If substantiated, this finding may offer an alternative cytapheresis schedule for donors.

中文翻译：

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在延长 G-CSF 递送期间同基因供体的双相 CD34+ 细胞动员

背景 文献中已经报道了超过 10 天的 G-CSF 给药和中性粒细胞单采术，但 CD34+ 细胞在这种情况下的动力学尚不清楚。案例 一名 42 岁女性因转移性乳腺癌接受了同基因移植。受者在移植前处于危急状态，因此供者接受了 13 天的 G-CSF，在此期间对 PBPC 和中性粒细胞进行了 8 次细胞分离。注意到 CD34+ 细胞中有两个峰；第一次在第 5 天和第二次在 G-CSF 给药的第 10-13 天；总共输注了 11.6 x 10(6)/kg CD34+ 细胞和 38.11 x 10(8)/kg 中性粒细胞。接受者的 ANC 在第 +3 天超过 0.1 x 10(9)/L。讨论 据我们所知，这是来自正常供体的 CD34+ 细胞的最长报道的细胞分离术。细胞分离术产品中的双相模式也令人感兴趣。这是一种不寻常的模式，表明骨髓祖细胞库发生了深刻而复杂的改变。如果得到证实，这一发现可能会为捐赠者提供替代的细胞单采计划。