In the present study, we have analyzed the effect of chronic amphetamine sulfate (AMPH) treatment on haematological, immunological and neurochemical parameters in the male rat. AMPH increased the total peripheral leukocyte count, and altered its differential counts, decreasing lymphocytes and increasing neutrophils. Flow cytometry study showed that the decline in circulating lymphocytes was caused by the loss of a particular lymphocyte subset, B-cell, which reduced both in percentage and in absolute number by 50%. T-cell population increased by 15% but not in absolute number, however there was no difference in either CD4+ or CD8+ T lymphocyte subsets between experimental groups. Neurochemically, AMPH reduced norepinephrine (NE) and serotonin (5-HT) contents in the hypothalamus and increased dopamine (DA) content in the striatum. Chronic AMPH increased in a dose-dependent manner serum corticosterone levels, had no effect on circulating catecholamines, reduced adrenal weights, and did not affect spleen weights although reduced their cellularities. These results show that chronic AMPH have important effects on immune function, particularly on humoral immune response because it reduced the circulating B cell population by half. In addition, AMPH plays an important role in the redistribution and trafficking of leukocytes, and both effects seem to be mediated by sympathetic innervation of the lymphoid organs.

中文翻译：

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慢性苯丙胺治疗对雄性大鼠的血液学、免疫学和神经化学影响。

在本研究中，我们分析了慢性硫酸苯丙胺 (AMPH) 治疗对雄性大鼠血液学、免疫学和神经化学参数的影响。AMPH 增加总外周白细胞计数，并改变其分类计数，减少淋巴细胞和增加中性粒细胞。流式细胞术研究表明，循环淋巴细胞的减少是由特定淋巴细胞亚群 B 细胞的丢失引起的，该亚群的百分比和绝对数量均减少了 50%。T 细胞群增加了 15%，但不是绝对数量，但实验组之间的 CD4+ 或 CD8+ T 淋巴细胞亚群没有差异。在神经化学上，AMPH 降低了下丘脑中的去甲肾上腺素 (NE) 和血清素 (5-HT) 含量，并增加了纹状体中的多巴胺 (DA) 含量。慢性 AMPH 以剂量依赖性方式增加血清皮质酮水平，对循环儿茶酚胺没有影响，减少肾上腺重量，虽然减少了脾脏的细胞结构，但不影响脾脏重量。这些结果表明慢性 AMPH 对免疫功能有重要影响，特别是对体液免疫反应，因为它使循环 B 细胞数量减少了一半。此外，AMPH 在白细胞的重新分布和运输中起着重要作用，这两种作用似乎都由淋巴器官的交感神经支配介导。这些结果表明，慢性 AMPH 对免疫功能具有重要影响，特别是对体液免疫反应，因为它使循环 B 细胞数量减少了一半。此外，AMPH 在白细胞的重新分布和运输中起着重要作用，这两种作用似乎都由淋巴器官的交感神经支配介导。这些结果表明，慢性 AMPH 对免疫功能具有重要影响，特别是对体液免疫反应，因为它使循环 B 细胞数量减少了一半。此外，AMPH 在白细胞的重新分布和运输中起着重要作用，这两种作用似乎都由淋巴器官的交感神经支配介导。