DNA-microarray technology is a powerful tool to explore the potentials of functional genomics. Almost a decade back, it was not so easy to trace the molecular pathway process in the pathogenesis of diseases. Today DNA-microarray technology allows identifying candidate genes that are implicated in the development and progression of diseases. Because this technology is new, it remains a challenge to perform DNA-microarray experiments in the laboratory. All the technical factors associated with DNA-microarray experiments have a strong impact on the results and therefore, all parts/steps of the protocol, in particular sample preparation (i.e., RNA isolation, RNA preamplification, and cDNA labeling), hybridization, washing, and scanning settings need to be checked and optimized to obtain reliable results from DNA-microarray experiments.

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DNA-微阵列技术：比较近期技术对基因表达谱的方法学因素。

DNA芯片技术是探索功能基因组学潜力的强大工具。大约十年前，在疾病的发病机理中追踪分子途径过程并不容易。如今，DNA芯片技术可以识别与疾病发展和进程有关的候选基因。由于这项技术是新技术，因此在实验室中进行DNA微阵列实验仍然是一项挑战。与DNA微阵列实验相关的所有技术因素均会对结果产生重大影响，因此，协议的所有部分/步骤，特别是样品制备（即RNA分离，RNA前置扩增和cDNA标记），杂交，洗涤，需要检查和优化扫描设置，以从DNA微阵列实验中获得可靠的结果。