Colletogloeopsis zuluensis, previously known as Coniothyrium zuluense, causes a serious stem canker disease on Eucalyptus spp. grown as non-natives in many tropical and sub-tropical countries. This stem canker disease was first reported from South Africa and it has subsequently been found on various species and hybrids of Eucalyptus in other African countries as well as in countries of South America and South-East Asia. In previous studies, phylogenetic analyses based on DNA sequence data of the ITS region suggested that all material of C. zuluensis was monophyletic. However, the occurrence of the fungus in a greater number of countries, and analyses of DNA sequences with additional isolates has challenged the notion that a single species is involved with Coniothyrium canker. The aim of this study was to consider the phylogenetic relationships amongst C. zuluensis isolates from all available locations and to support these analyses with phenotypic and morphological comparisons. Individual and combined phylogenies were constructed using DNA sequences from the ITS region, exons 3 through 6 of the beta-tubulin gene, the intron of the translation elongation factor 1-alpha gene, and a partial sequence of the mitochondrial ATPase 6 gene. Both phylogenetic data and morphological characteristics showed clearly that isolates of C. zuluensis represent at least two taxa. One of these is C. zuluensis as it was originally described from South Africa, and we provide an epitype for it. The second species occurs in Argentina and Uruguay, and is newly described as C. gauchensis. Both fungi are serious pathogens resulting in identical symptoms. Recognising them as different species has important quarantine consequences.

中文翻译：

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多基因系统发育和表型特征区分了与桉树茎溃疡相关的 Colletogloeopsis zuluensis 复合体中的两个物种。

Colletogloeopsis zuluensis，以前称为 Coniothyrium zuluense，在桉树属上引起严重的茎溃疡病。在许多热带和亚热带国家作为非本地人种植。这种茎溃疡病首先在南非报道，随后在其他非洲国家以及南美洲和东南亚国家的桉树的各种物种和杂交种上发现。在以往的研究中，基于 ITS 区域 DNA 序列数据的系统发育分析表明，C. zuluensis 的所有材料都是单系的。然而，该真菌在更多国家的出现，以及对其他分离株的 DNA 序列分析，对单一物种与 Coniothyrium 溃疡病有关的观点提出了挑战。本研究的目的是考虑来自所有可用位置的 C. zuluensis 分离株之间的系统发育关系，并通过表型和形态比较支持这些分析。使用来自 ITS 区域的 DNA 序列、β-微管蛋白基因的外显子 3 到 6、翻译延伸因子 1-α 基因的内含子和线粒体 ATPase 6 基因的部分序列构建了个体和组合系统发育。系统发育数据和形态特征都清楚地表明 C. zuluensis 的分离物代表至少两个分类群。其中之一是 C. zuluensis，因为它最初是从南非描述的，我们为它提供了一个表型。第二个物种出现在阿根廷和乌拉圭，新描述为 C. gauchensis。这两种真菌都是导致相同症状的严重病原体。将它们识别为不同的物种具有重要的检疫后果。