Three strains of sulfate-reducing bacteria (ADR21, ADR26 and ADR28) were isolated from Adour estuary sediments (French South Atlantic coast). Cells of these isolates were rod-shaped, motile and stained Gram-negative. The 16S rRNA and dsrAB genes sequence analyses indicated that these three strains belonged to the genus Desulfomicrobium within the delta Proteobacteria, with Desulfomicrobium escambiense strain DSM10707T as their closest relative. According to phenotypic characteristics, strains ADR21 and ADR28 could be considered as members of the same species. The relatedness values, based on DNA-DNA hybridization studies, between strains ADR21/DSM10707T, ADR26/DSM10707T and ADR21/ADR26 ranged between 30.6-40.8%, 45.2-43.0% and 19.0-26.4%, respectively. Strains ADR21 and ADR28 grew well on lactate, fumarate, malate, formate, ethanol and H2/acetate in the presence of sulfate as an electron acceptor. Thiosulfate, nitrate, fumarate and DMSO were alternative electron acceptors. Malate was well fermented but pyruvate and fumarate only poorly. Strain ADR26 could not grow on ethanol or fumarate and was unable to use DMSO or fumarate as electron acceptors. The three new strains exhibited differences compared to the type strain of D. escambiense, such as temperature optima, substrate utilization and mercury methylation capacities. On the basis of both genetic and phenotypic evidences, strain ADR21 is proposed as the type strain of the species Desulfomicrobium salsuginis sp. nov., and strain ADR26 as the type strain of the species Desulfomicrobium aestuarii sp. nov.

中文翻译：

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Desulfomicrobium salsuginis sp。的特征。十一月 和Desulfomicrobium aestuarii sp。十一月，从Adour河口（法国大西洋海岸）分离出的两种新的硫酸盐还原细菌，它们具有特定的汞甲基化潜力。

从Adour河口沉积物（法国南大西洋海岸）中分离出三株减少硫酸盐的细菌（ADR21，ADR26和ADR28）。这些分离物的细胞呈杆状，能动且革兰氏阴性。16S rRNA和dsrAB基因序列分析表明，这三个菌株均属于三角洲变形杆菌内的Desulfomicrobium属，而Esambiambiense Desulfomicrobium escambiense菌株DSM10707T为它们的近亲。根据表型特征，菌株ADR21和ADR28可以被视为同一物种的成员。基于DNA-DNA杂交研究，菌株ADR21 / DSM10707T，ADR26 / DSM10707T和ADR21 / ADR26之间的相关性值分别在30.6-40.8％，45.2-43.0％和19.0-26.4％之间。菌株ADR21和ADR28在乳酸，富马酸酯，苹果酸，甲酸盐，硫酸盐作为电子受体存在下，乙醇和H2 /乙酸盐。硫代硫酸盐，硝酸盐，富马酸盐和DMSO是替代电子受体。苹果酸发酵得很好，但是丙酮酸和富马酸酯的发酵能力很差。菌株ADR26不能在乙醇或富马酸酯上生长，并且不能使用DMSO或富马酸酯作为电子受体。这三种新菌株与D. escambiense菌株相比表现出差异，例如温度最佳，底物利用率和汞甲基化能力。根据遗传和表型证据，拟定菌株ADR21作为Salsuginis sp。菌种的类型菌株。No.，并且将菌株ADR26作为种Desulfomicrobium aestuarii sp。的类型菌株。十一月 富马酸酯和DMSO是替代电子受体。苹果酸发酵得很好，但是丙酮酸和富马酸酯的发酵能力很差。菌株ADR26不能在乙醇或富马酸盐上生长，并且不能使用DMSO或富马酸盐作为电子受体。这三种新菌株与D. escambiense菌株相比表现出差异，例如温度最佳，底物利用率和汞甲基化能力。根据遗传和表型证据，拟定菌株ADR21作为Salsuginis sp。菌种的类型菌株。No.，并且将菌株ADR26作为种Desulfomicrobium aestuarii sp。的类型菌株。十一月 富马酸酯和DMSO是替代电子受体。苹果酸发酵得很好，但是丙酮酸和富马酸酯的发酵能力很差。菌株ADR26不能在乙醇或富马酸酯上生长，并且不能使用DMSO或富马酸酯作为电子受体。这三种新菌株与D. escambiense菌株相比表现出差异，例如温度最佳，底物利用率和汞甲基化能力。根据遗传和表型证据，拟定菌株ADR21作为Salsuginis sp。菌种的类型菌株。No.，并且将菌株ADR26作为种Desulfomicrobium aestuarii sp。的类型菌株。十一月 这三种新菌株与D. escambiense菌株相比表现出差异，例如温度最佳，底物利用率和汞甲基化能力。根据遗传和表型证据，拟定菌株ADR21作为Salsuginis sp。菌种的类型菌株。No.，并且将菌株ADR26作为种Desulfomicrobium aestuarii sp。的类型菌株。十一月 这三种新菌株与D. escambiense菌株相比表现出差异，例如温度最佳，底物利用率和汞甲基化能力。根据遗传和表型证据，提出菌株ADR21作为Salsuginis sp。的物种。No.，并且将菌株ADR26作为种Desulfomicrobium aestuarii sp。的类型菌株。十一月