Vitamin C (L-ascorbic acid; AsA) is the major soluble antioxidant found in plants and is also an essential component of human nutrition. Although numerous biotechnological methods have been exploited to increase its yield, pressures such as commercial competition and environmental concerns make it urgent to find a new way for industrial production of plant-derived AsA. Engineering plant AsA has now become feasible because of our increased understanding of its biosynthetic pathway. Several possible strategies could be followed to increase AsA production, such as overcoming the rate limiting steps in the biosynthetic pathway, promoting recycling, and reducing catabolism. For these purposes, genes of plant, microbial and animal origins have been successfully used. Several examples will be given to illustrate these various approaches. The existing and potential achievements in increasing AsA production would provide the opportunity for enhancing nutritional quality and stress tolerance of crop plants.

中文翻译：

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植物L-抗坏血酸生物合成的代谢工程：最新趋势和应用。

维生素C（L-抗坏血酸； AsA）是植物中发现的主要可溶性抗氧化剂，也是人体营养的必需成分。尽管已经开发了许多生物技术方法来提高其产量，但是诸如商业竞争和环境问题等压力使得迫切需要寻找一种工业化生产植物性AsA的新方法。由于我们对其生物合成途径的更多了解，工程设备AsA现在变得可行。可以采取几种可能的策略来增加AsA的产生，例如克服生物合成途径中的限速步骤，促进回收利用和减少分解代谢。为了这些目的，已经成功地使用了植物，微生物和动物来源的基因。将给出几个示例来说明这些各种方法。