This paper proposes a nanopore-based sensor exploiting the solution exchange of a droplet-based lipid bilayer driven by a superabsorbent polymer. Biological nanopores are candidates for use in portable sensors because of their potential to recognize and detect single molecules. One of the current challenges in the development of portable nanopore sensors is the inability to achieve continuous detection. To achieve continuous detection, we have exploited the suction force of a superabsorbent polymer to drive the continuous microfluidic flow required to wash the analyte out of the droplet. The superabsorbent polymer drives the microfluidic flow without electricity, and the developed solution exchange system remains compact. To demonstrate solution exchange in the droplet containing the lipid bilayer, the concentration of heptakis(6-O-sulfo)-β-cyclodextrin was monitored in a time-dependent manner using α-hemolysin nanopores. A reduction in the concentration, attributable to solution exchange, was successfully observed. We believe that the proposed system will increase the portability and usability of nanopore sensors.

中文翻译：

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用于纳米孔传感器的无泵溶液交换系统。

本文提出了一种基于纳米孔的传感器，该传感器利用了由超吸收性聚合物驱动的基于液滴的脂质双层的溶液交换。生物纳米孔因其识别和检测单个分子的潜力而成为便携式传感器的候选者。便携式纳米孔传感器开发中的当前挑战之一是无法实现连续检测。为了实现连续检测，我们利用了超吸收性聚合物的吸力来驱动从微滴中洗出分析物所需的连续微流。超吸收性聚合物无需电即可驱动微流体流动，并且开发的溶液交换系统保持紧凑。为了证明溶液在含有脂质双层的液滴中的交换，使用α-溶血素纳米孔以时间依赖性方式监测七（6-O-磺基）-β-环糊精的浓度。成功观察到归因于溶液交换的浓度降低。我们相信，提出的系统将增加纳米孔传感器的便携性和可用性。