BACKGROUND The molecular mechanisms underlying the biologic effects or differentiation of mesenchymal stromal cells (MSC) have not been clarified. Screening for genes differentially expressed at different stages is an important step in determining these molecular mechanisms. METHODS In this study, we analyzed the gene expression profiles of C3H10T1/2 (10T1/2) cells and two sublines, A54 (pre-adipocyte) and M1601 (myoblast), as a model of MSC and downstream committed progenitors. RESULTS We found up-regulated expression of delta-like-1 (Dlk), Wnt-5a and IL-1 receptor-like-1 (ST2) in 10T1/2 cells; stem cell factor (SCF) and stromal derived factor-1 (SDF-1) in A54 cells; and cardiac muscle-specific gene in M1601 cells. Overexpression of Dlk in A54 cells did not induce any effects on their differentiation into adipocytes. After differentiation into adipocytes, A54 cells reduced the expression of SCF, SDF-1 and Ang-1 as well as the ability to support the formation of a cobblestone appearance. DISCUSSION The results suggest that these three lines hae different gene profiles and are a useful system for analyzing the differentiation and function of MSC and progenitor cells.

中文翻译：

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通过 C3H10T1/2 和 C3H10T1/2 衍生细胞系的 DNA 微阵列分析筛选负责小鼠间充质基质细胞分化的基因

背景间充质基质细胞（MSC）的生物学效应或分化的分子机制尚未阐明。筛选在不同阶段差异表达的基因是确定这些分子机制的重要步骤。方法 在本研究中，我们分析了 C3H10T1/2 (10T1/2) 细胞和两个亚系 A54（前脂肪细胞）和 M1601（成肌细胞）的基因表达谱，作为 MSC 和下游定向祖细胞的模型。结果 我们发现 10T1/2 细胞中 delta-like-1 (Dlk)、Wnt-5a 和 IL-1 receptor-like-1 (ST2) 的表达上调；A54 细胞中的干细胞因子 (SCF) 和基质衍生因子-1 (SDF-1)；M1601 细胞中的心肌特异性基因。Dlk 在 A54 细胞中的过度表达不会对其分化为脂肪细胞产生任何影响。分化为脂肪细胞后，A54 细胞降低了 SCF、SDF-1 和 Ang-1 的表达以及支持鹅卵石外观形成的能力。讨论 结果表明，这三个细胞系具有不同的基因谱，是分析 MSC 和祖细胞的分化和功能的有用系统。