Directed evolution is being used increasingly in industrial and academic laboratories to modify and improve commercially important enzymes. Laboratory evolution is thought to make its biggest contribution in explorations of non-natural functions, by allowing us to distinguish the properties nurtured by evolution. In this review we report the significant advances achieved with respect to the methods of biocatalyst improvement and some critical properties and applications of the modified enzymes. The application of directed evolution has been elaborately demonstrated for protein solubility, stability and catalytic efficiency. Modification of certain enzymes for their application in enantioselective catalysis has also been elucidated. By providing a simple and reliable route to enzyme improvement, directed evolution has emerged as a key technology for enzyme engineering and biocatalysis.

中文翻译：

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定向进化：一种工程化酶的方法。

在工业和学术实验室中，越来越多的人采用定向进化来修饰和改进商业上重要的酶。人们认为，实验室进化通过允许我们区分进化所培养的特性，在非自然功能探索中做出了最大贡献。在这篇综述中，我们报告了在生物催化剂改进方法以及修饰酶的一些关键特性和应用方面所取得的重大进展。对于蛋白质的溶解度，稳定性和催化效率，已经详细证明了定向进化的应用。还阐明了某些酶在对映选择性催化中的应用修饰。通过提供简单可靠的酶改良途径，