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Proteome-scale analysis of biochemical activity.
Critical Reviews in Biochemistry and Molecular Biology ( IF 6.2 ) Pub Date : 2006-08-17 , DOI: 10.1080/10409230600872872
Eric M Phizicky 1 , Elizabeth J Grayhack
Affiliation  

During the last 10 years, there has been a large increase in the number of genome sequences available for study, altering the way that the biology of organisms is studied. In particular, scientific attention has increasingly focused on the proteome, and specifically on the role of all the proteins encoded by the genome. We focus here on several aspects of this problem. We describe several technologies in widespread use to clone genes on a genome-wide scale, and to express and purify the proteins encoded by these genes. We also describe a number of methods that have been developed to analyze various biochemical properties of the proteins, with attention to the methodology and the limitations of the approaches, followed by a look at possible developments in the next decade.

中文翻译:

蛋白质组规模的生化活性分析。

在过去的十年中,可用于研究的基因组序列的数量大大增加,从而改变了研究生物生物学的方式。特别地,科学的注意力越来越集中在蛋白质组上,尤其是基因组编码的所有蛋白质的作用上。我们在这里集中讨论此问题的几个方面。我们描述了几种广泛用于克隆全基因组范围内的基因,以及表达和纯化这些基因编码的蛋白质的技术。我们还描述了已开发出的多种方法,用于分析蛋白质的各种生化特性,同时关注方法学和方法的局限性,然后着眼于未来十年的可能发展。
更新日期:2019-11-01
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