The lysosomal membrane stability test applied on the digestive cells (LMS) and the neutral red lysosomal retention assay (NRR) performed on hemocytes have been evaluated on mussels Mytilus galloprovincialis collected from Thermaikos and Strymonikos gulfs (Nothern Greece) in June and December 2000. The correlation analysis indicated that there was a strong positive relationship between the above mentioned biomarkers in both sampling seasons, supporting the validity of their application in water quality assessment. The evaluation of micronucleus frequency (MN) has been also performed in hemocytes of the same mussels and, according to the results, it requires additional validation before using it as a stress index. The results obtained in parallel by the stress on stress (SOS) technique do not support the application of this biomarker in biomonitoring, showing, however, negative correlation with LMS and NRR in one sampling season. Finally, morphological observations were performed on cryosections stained for the enzyme N-acetyl-beta-hexosaminidase, revealing differences in the epithelial cell-layer thickness, as well as changes in the digestive lysosomal system of mussels, obtained from different sampling sites in the two sampling seasons.

中文翻译：

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希腊海岸的Mytilus galloprovincialis中的多个生物标志物比较：“溶酶体膜稳定性，中性红色保留，微核频率和压力胁迫”。

已对2000年6月和12月从Thermaikos和Strymonikos海湾（希腊北部）收集的贻贝Mytilus galloprovincialis进行了评估，将其应用于消化细胞（LMS）的溶酶体膜稳定性测试和对血细胞进行的中性红溶酶体保留测定（NRR）。相关分析表明，在两个采样季节中，上述生物标志物之间都存在很强的正相关关系，这支持了它们在水质评估中的有效性。还已经在相同贻贝的血细胞中进行了微核频率（MN）的评估，根据结果，在将其用作压力指标之前，还需要进行其他验证。通过胁迫（SOS）技术并行获得的结果不支持该生物标记物在生物监测中的应用，但是，显示在一个采样季节中LMS和NRR呈负相关。最后，对染色后的N-乙酰-β-己糖胺酶冷冻切片进行形态学观察，揭示了贻贝上皮细胞层厚度的差异以及消化溶酶体系统的变化，这是从两个采样点获得的采样季节。