To study the role of Cd-induced phytochelatins in UV-B tolerance, lipid peroxidation, antioxidative enzymes (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase), glutathione arid phytochelatin contents were measured in buthionine sulphoximine treated and untreated cells of Anabaena doliolum. Cd-pretreatment of the cyanobacterium reduced the lipid peroxidation as well as the antioxidative enzymes in comparison to UV-B treatment alone, whereas the phytochelatin content demonstrated an increase. In contrast to this, buthionine sulphoximine-induced inhibition of phytochelatin synthase, dramatically decreased the Cd-induced co-tolerance against UV-B, hence demonstrating that phytochelatin not only protects the cyanobacterium from heavy metal but participates in UV-B tolerance as well.

中文翻译：

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植物螯合素在固氮蓝藻 Anabaena doliolum 的 UV-B 耐受性中起作用

为了研究 Cd 诱导的植物螯合素在 UV-B 耐受性、脂质过氧化、抗氧化酶（超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶和谷胱甘肽还原酶）中的作用，在丁硫氨酸亚砜亚胺处理和未处理的鱼腥藻细胞中测量谷胱甘肽和植物螯合素含量。与单独的 UV-B 处理相比，蓝藻的 Cd 预处理减少了脂质过氧化以及抗氧化酶，而植物螯合素含量显示出增加。与此相反，丁硫氨酸亚砜亚胺诱导的植物螯合素合酶抑制显着降低了 Cd 诱导的对 UV-B 的共耐受性，因此表明植物螯合素不仅保护蓝藻免受重金属的侵害，而且还参与了 UV-B 耐受性。