RNA-mediated interference (RNAi) was employed to systematically inactivate the four subunits of complex II in the mitochondrial electron transport chain. Embryonic lethality was the predominant result of inactivating three subunits (ceSDHB, ceSDHC, and ceSDHD) when using the soaking method to inactivate RNA. The feeding method was employed to deliver dsRNA from the fourth subunit (ceSDHA) to wild-type, mev-1 (mutated in ceSDHC of complex II), and gas-1 animals (mutated in a complex I gene). Survival was reduced only in the mev-1 genetic background, and in an oxygen-dependent fashion. Collectively, these data provide further evidence that compromised complex II integrity can result in sensitivity to oxidative stress.

中文翻译：

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复合物 II 灭活在线虫秀丽隐杆线虫中是致命的

RNA 介导的干扰 (RNAi) 被用来系统地灭活线粒体电子传递链中复合物 II 的四个亚基。当使用浸泡方法灭活 RNA 时，胚胎致死率是三个亚基（ceSDHB、ceSDHC 和 ceSDHD）灭活的主要结果。采用喂养方法将来自第四亚基 (ceSDHA) 的 dsRNA 递送至野生型、mev-1（在复合物 II 的 ceSDHC 中发生突变）和 gas-1 动物（在复合物 I 基因中发生突变）。仅在 mev-1 遗传背景中，存活率降低，并且依赖于氧气。总的来说，这些数据提供了进一步的证据，证明复合物 II 完整性受损会导致对氧化应激的敏感性。