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t-Loops in yeast mitochondria
Mitochondrion ( IF 4.4 ) Pub Date : 2002-05-01 , DOI: 10.1016/s1567-7249(02)00009-0
Lubomir Tomaska 1 , Alexander M Makhov , Jack D Griffith , Jozef Nosek
Affiliation  

Mitochondria of several yeast species contain a linear DNA genome possessing specific terminal DNA structures dubbed mitochondrial telomeres. Several tandemly repeated units and a 5' single-stranded extension characterize mitochondrial telomeres in Candida parapsilosis, Pichia philodendra and Candida salmanticensis. Resemblance of this type of mitochondrial telomeres to typical nuclear telomeres suggests that they might form t-loop structures. Therefore we adopted a protocol for stabilization of potential t-loops in the mtDNA of C. parapsilosis and observed several loops at the ends of the mtDNA. A potential role of t-loops in protection of the ends of mtDNA and/or in mitochondrial telomere dynamics is discussed.

中文翻译:

酵母线粒体中的 t 环

几种酵母物种的线粒体包含一个线性 DNA 基因组,该基因组具有称为线粒体端粒的特定末端 DNA 结构。几个串联重复单元和 5' 单链延伸表征近平滑念珠菌、Pichia philodendra 和 Candida salmanticensis 中的线粒体端粒。这种类型的线粒体端粒与典型的核端粒相似,表明它们可能形成 t 环结构。因此,我们采用了一种稳定 C. parapsilosis 的 mtDNA 中潜在 t 环的协议,并在 mtDNA 的末端观察了几个环。讨论了 t 环在保护 mtDNA 末端和/或线粒体端粒动力学中的潜在作用。
更新日期:2002-05-01
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