Contamination of small-grain cereals with the fungal species Fusarium graminearum, F. culmorum, F. poae, F. sporotrichioides and F. equiseti is an important source of trichothecenes, Zearalenone and other mycotoxins which cause serious diseases in human and animals. Additionally, these species contribute to Fusarium Head Blight, a disease which produces important losses in cereal yield. Early detection and control of these Fusarium species is crucial to prevent toxins entering the food chain and a useful tool in disease management practices. We describe the development of specific PCR assays to F. graminearum, F. culmorum, F. poae, F. sporotrichioides and F. equiseti using DNA from pure fungal cultures as well as from naturally infected wheat seeds, using in this case a rapid and easy protocol for DNA isolation. The specific primers were designed on the basis of IGS sequences (Intergenic Spacer of rDNA), a multicopy region in the genome that permits to enhance the sensitivity of the assay in comparison with PCR assays based on single-copy sequences.

中文翻译：

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PCR检测方法用于生产天花粉烯的禾本科镰刀菌，镰刀镰刀菌，镰刀菌，马齿镰刀菌和孢子镰刀菌。

小谷类谷物被真菌镰刀镰刀菌，细角镰刀菌，细角镰刀菌，细孢镰刀菌和木贼镰刀菌污染是毛孢菌毒素，玉米赤霉烯酮和其他霉菌毒素的重要来源，会导致人类和动物的严重疾病。此外，这些物种还导致镰刀菌枯萎病，这种疾病导致谷物单产严重下降。这些镰刀菌属物种的早期发现和控制对于防止毒素进入食物链至关重要，也是疾病管理实践中的有用工具。我们描述了使用纯真菌培养以及天然感染的小麦种子中的DNA对禾本科禾本科植物，禾本科植物，禾本科植物，禾本科孢子虫和马鞭草的特异性PCR分析方法的开发，在这种情况下使用快速和简便的DNA分离方案。