Mauritius is one of the largest world producers of Anthurium cut flowers but outbreaks of bacterial blight have never been reported on the island. This work was about the characterisation and identification of bacterial strains isolated from Anthurium andreanum, Dieffenbachia maculata and Aglaonema simplex in Mauritius. Fifteen strains, that showed the morphological properties of Xanthomonas on conventional media, were tested on two semi-selective media (Esculin-trehalose and cellobiose-starch). ELISA tests using a panel of monoclonal antibodies were carried out and three out of 15 strains reacted with a Xanthomonas-specific monoclonal antibody (MAb XII). Analysis using four sets of ribosomal primers revealed that the same three Mauritius strains shared conserved PCR products with reference xanthomonads including virulent strains of Xanthomonas axonopodis pv. dieffenbachiae (Xad). BIOLOG tests and the Sherlock Microbial Identification system (MIDI) identified these three new strains at the species level as X. axonopodis. The complementary tests that were carried out clearly confirmed that the three strains are xanthomonads and, moreover, a DNA probe which showed specificity to Xad strains suggested that the three Mauritius strains are non-virulent forms of the pathogen causing Anthurium blight.

中文翻译：

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从毛里求斯的小行星分离的黄单胞菌菌株的分子表征。

毛里求斯是世界上最大的红掌生产国之一，但岛上从未有细菌疫病暴发的报道。这项工作是关于表征和鉴定从毛里求斯的红掌，金叶万年青和简单的Aglaonema分离的细菌菌株。在两种半选择性培养基（Esculin-海藻糖和纤维二糖-淀粉）上测试了十五种在常规培养基上显示黄单胞菌的形态特性的菌株。使用一组单克隆抗体进行了ELISA测试，每15个菌株中就有3个与Xanthomonas特异性单克隆抗体（MAb XII）反应。使用四套核糖体引物进行的分析显示，相同的三个毛里求斯菌株与包括黄原体强毒株的强毒株在内的参比黄原菌共享保守的PCR产物。双芬氏菌（Xad）。BIOLOG测试和Sherlock微生物鉴定系统（MIDI）在物种水平上将这三个新菌株鉴定为X. axonopodis。进行的补充试验清楚地证实了这三个菌株是黄单胞菌，此外，对Xad菌株表现出特异性的DNA探针表明这三个毛里求斯菌株是引起红掌疫病的病原体的非毒性形式。