A Gram-negative bacterium designated AC-49T was isolated from an alkaline groundwater with a pH 11.4. This organism formed rod-shaped cells, was strictly aerobic, catalase and oxidase positive, with an optimum growth temperature of 35 degrees C and an optimum pH value of 8.0. Strain AC-49T assimilated primarily amino acids and some Krebs cycle metabolites, did not use sugars for growth. The organism did not grow on L-phenylalanine or antipyrin. The G+ C content of DNA was 66.9 mol%. The phylogenetic analyses based on the 16S rRNA sequencing showed that the closest relatives of strain AC-49T were Phenylobacterium lituiforme and Phenylobacterium immobile, indicating that the organism is a member of the order Caulobacterales of the Alphaproteobacteria. Based on the phylogenetic analyses and distinct phenotypic characteristics, we are of the opinion that strain AC-49T, represents a novel species of the genus Phenylobacterium for which we propose the name Phenylobacterium falsum sp. nov.

中文翻译：

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镰刀菌 nov。，一种从非盐碱地下水中分离出的Alteproteobacter细菌，并改良了Phenylobacterium属的描述。

从pH 11.4的碱性地下水中分离出革兰氏阴性细菌AC-49T。该生物形成杆状细胞，严格需氧，过氧化氢酶和氧化酶呈阳性，最适生长温度为35摄氏度，最适pH值为8.0。菌株AC-49T主要吸收氨基酸和一些Krebs循环代谢物，没有使用糖来生长。该生物体未在L-苯丙氨酸或安替比林上生长。DNA的G + C含量为66.9mol％。基于16S rRNA测序的系统发育分析表明，菌株AC-49T的最亲近的亲戚是枯萎苯基苯丙酸杆菌和固定不动的苯基苯丙酸杆菌，这表明该生物体是丙酸杆菌的杆状细菌的成员。根据系统发育分析和独特的表型特征，我们认为，菌株AC-49T代表了苯酚杆菌属的一个新物种，为此我们将其命名为falsum sp.。十一月