Existence of stem cells in adult pancreas remains contentious. Single cells suspensions obtained by collagenase and trypsin digestion separately from adult mouse pancreas and pancreatic islets were spun at 1000 rpm (250 g) to collect the cells. At this speed the stem/ progenitor cells remained buoyant and were further enriched by spinning the supernatant at 3000 rpm (1000 g). Two distinct populations of stem cells were detected including pluripotent, very small (2–6 μm) embryonic-like stem cells (VSELs) that expressed nuclear OCT-4A and pluripotent transcripts (Oct-4A, Sox2, Nanog, Stella) and slightly bigger progenitors, pancreatic stem cells (PSCs) that expressed cytoplasmic OCT-4B and PDX-1. Streptozotocin treated diabetic pancreas showed an increase in numbers of VSELs (2–6 μm, 7AAD-, LIN-CD45-SCA1+ cells) and up-regulation of transcripts specific for stem/ progenitor cells. Diabetic mice were further subjected to partial pancreatectomy to study involvement of VSELs/ PSCs during regeneration. VSELs/ PSCs were mobilized in large numbers, were observed in the lumen of blood vessels and PCNA expression suggested their proliferation. Initially, new acini assembled to regenerate the exocrine pancreas and later by Day 30, neogenesis of islets was observed in the vicinity of the blood vessels and pancreatic ducts by the differentiation of endogenous VSELs/ PSCs which may be targeted to regenerate diabetic pancreas in clinical settings.

中文翻译：

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部分胰腺切除术后，小鼠的胰腺干细胞/祖细胞被链脲佐菌素增强并再生糖尿病胰腺。

成年胰腺中干细胞的存在仍然存在争议。通过胶原酶和胰蛋白酶消化分别从成年小鼠胰腺和胰岛中分离得到的单细胞悬液以1000 rpm（250 g）的转速离心收集细胞。以这种速度，干细胞/祖细胞保持漂浮状态，并通过以3000 rpm（1000 g）旋转上清液进一步富集。检测到两个不同的干细胞种群，包括表达核OCT-4A和多能转录本的多能，非常小（2–6μm）胚胎样干细胞（VSEL）（Oct-4A，Sox2，Nanog，Stella）以及稍大的祖细胞，即表达细胞质OCT-4B和PDX-1的胰腺干细胞（PSC）。链脲佐菌素治疗的糖尿病胰腺的VSEL数量（2–6μm，7AAD-，LIN-CD45-SCA1 +细胞）增加，并且干/祖细胞特异的转录物上调。糖尿病小鼠进一步接受部分胰腺切除术以研究再生过程中VSEL / PSC的参与。VSEL​​s / PSCs大量动员，在血管腔中观察到，PCNA表达提示其增殖。最初，组装新的腺泡以再生外分泌胰腺，然后到第30天，通过内源性VSEL / PSC的分化在血管和胰管附近观察到胰岛的新生，在临床环境中，这些VSEL / PSC可能靶向再生胰腺。