A novel moderately thermophilic, bacterium, strain SM250^T, was isolated from a terrestrial mud volcano, Taman peninsula, Krasnodar region, Russia. Cells of strain SM250^T were Gram-negative non-spore forming motile straight rods. Growth was observed at temperatures 30–63 °C (optimum at 50 °C), pH 6.5–10.0 (optimum at pH 8.5) and NaCl concentrations 0–4.5% (w/v) (optimum at 1.0–1.5% (w/v)). The novel isolate grows by aerobic respiration or anaerobic respiration with nitrate as the terminal electron acceptor. Strain SM250^T grows by the utilization of methanol, formate and a number of other organic compounds or lithoautotrophically with hydrogen, elemental sulfur or thiosulfate as electron donors. The total size of the genome of the novel isolate was 3,327,116 bp and a genomic DNA G + C content was 64.8 mol%. Analysis of the 16S rRNA gene sequences revealed that strain SM250^T belongs to the class Hydrogenophilia within the phylum Proteobacteria, with less than 91% of 16S rRNA gene sequence similarity to any species with validly published name. We propose to assign strain SM250^T to a new species of a novel genus Pelomicrobium methylotrophicum gen. nov., sp. nov. The type strain is SM250^T (= KCTC 62861^T = VKM B-3274^T).

中文翻译：

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Pelomicrobiummethylotrophicum gen。十一月，sp。十一月 从陆生泥火山中分离出来的中等嗜热，兼性厌氧，自噬和甲基营养细菌。

一种新颖的中度嗜热，细菌，应变SM250 ^Ť，被从地面泥火山，塔曼半岛，克拉斯诺达区域，俄罗斯隔离。应变SM250的细胞^Ť是革兰氏阴性的非孢子形成游动直棒。在30-63°C（50°C时最适），pH 6.5-10.0（pH 8.5时最适）和NaCl浓度0-4.5％（w / v）（1.0-1.5％（w / v时最适） v））。该新型分离株通过需氧呼吸或以硝酸盐为末端电子受体的厌氧呼吸而生长。应变SM250 ^T通过利用甲醇，甲酸盐和许多其他有机化合物或通过自溶与氢，元素硫或硫代硫酸盐作为电子供体而自养。新分离物的基因组总大小为3,327,116 bp，基因组DNA G + C含量为64.8 mol％。16S rRNA基因序列的分析表明，菌株SM250 ^Ť属于类Hydrogenophilia的内门变形菌，用16S rRNA基因的序列相似性小于91％的与合格发表名称的任何物种。我们建议将菌株SM250 ^T分配给一种新的Pelomicrobiummethylotrophicum属。十一月，sp。十一月 类型应变为SM250 ^T（= KCTC 62861^T  = VKM B-3274 ^T）。