2-Hydroxymelatonin (2-OHMT) is an important metabolite produced through melatonin interaction with oxygenated compounds. 2-OHMT pretreated seeds (50 µM, 100 µM, and 150 µM) were grown in soil contaminated with 50 mg kg-1 cadmium. Cadmium imposed stress reduced seed germination, growth, biomass production, and chlorophyll (Chl) content in Cucumis sativus seedlings. 2-OHMT application emphatically revamped germination, shoot length, root length, and plant biomass production. The 2-OHMT pretreatment modulated expression levels of plasma membrane H+-ATPase genes of C. sativus including CsHA2, CsHA3, CsHA4, CsHA8, and CsHA9. This biomolecule amplified the accumulation of antioxidants such as glutathione, proline, phenolics, and flavonoids. The reduced Cd-uptake in 2-OHMT treated C. sativus seedlings encouraged uptake of essential plant nutrients. Furthermore, conjugated increase of indole acetic acid contents and ethylene production rate were observed in 2-OHMT treated seedlings in a dose-dependent manner. The improved nutritional content in 2-OHMT applied seedlings was ascribed to enhanced expression of H+-ATPase regulating genes besides increased amount of non-enzymatic antioxidants in Cd-stressed plants. The present novel study elucidates the potential of 2-OHMT in improving nutritional content in cucumber plants by modulation of non-enzymatic antioxidants and gene expression.

中文翻译：

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2-羟基褪黑素诱导的黄瓜毒性对镉的营养协调作用：非酶抗氧化剂的调节和基因表达。

2-羟基褪黑激素（2-OHMT）是褪黑激素与含氧化合物相互作用产生的重要代谢产物。将2-OHMT预处理的种子（50 µM，100 µM和150 µM）种植在被50 mg kg-1镉污染的土壤中。镉胁迫降低了黄瓜黄瓜幼苗的种子发芽，生长，生物量生产和叶绿素（Chl）含量。2-OHMT的施用可显着改善发芽，枝条长，根长和植物生物量的产生。2-OHMT预处理调节了番茄的质膜H + -ATPase基因表达水平，包括CsHA2，CsHA3，CsHA4，CsHA8和CsHA9。这种生物分子放大了抗氧化剂如谷胱甘肽，脯氨酸，酚类和类黄酮的积累。在2-OHMT处理过的C. sativus幼苗中，Cd吸收减少，从而促进了植物必需养分的吸收。此外，在2-OHMT处理的幼苗中，吲哚乙酸含量和乙烯生产率的共轭增加是以剂量依赖的方式出现的。施用2-OHMT的幼苗中营养含量的提高归因于H + -ATPase调控基因的表达增强，此外镉胁迫植物中非酶抗氧化剂的含量增加。本新研究阐明了2-OHMT通过调节非酶性抗氧化剂和基因表达来提高黄瓜植物营养含量的潜力。施用2-OHMT的幼苗中营养含量的提高归因于H + -ATPase调控基因的表达增强，此外镉胁迫植物中非酶抗氧化剂的含量增加。本新研究阐明了2-OHMT通过调节非酶性抗氧化剂和基因表达来提高黄瓜植物营养含量的潜力。施用2-OHMT的幼苗中营养含量的提高归因于H + -ATPase调控基因的表达增强，此外镉胁迫植物中非酶抗氧化剂的含量增加。本新研究阐明了2-OHMT通过调节非酶性抗氧化剂和基因表达来提高黄瓜植物营养含量的潜力。