BACKGROUND/AIMS Bacteriophages (phages) are viruses of bacteria. Escherichia coli phage (T4) can potentially interfere with adsorption of HAdV-5 to cellular integrins by its KGD motif, while staphylococcal A5/80 phage does not possess this structure. The objective of this study was to investigate the effects of T4 and A5/80 phage preparations on type 5 human adenovirus (HAdV-5) DNA synthesis and the expression of HAdV-5 genes. METHODS Experiments were performed on the A549 cell line. HAdV-5 DNA synthesis was investigated with real-time PCR. Expression of HAdV-5 early (DBP) and late (hexon) genes was determined by quantitative real-time PCR in preincubation and coincubation experiments. RESULTS While both phage preparations significantly reduced the expression of HAdV-5 genes, synthesis of HAdV-5 DNA was inhibited only by T4. CONCLUSION Phage preparations show promise as novel antiviral agents. However, further studies are required to investigate their antiviral effects.

中文翻译：

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噬菌体制剂对腺病毒复制的抑制作用。

背景/目的噬菌体（噬菌体）是细菌的病毒。大肠杆菌噬菌体（T4）可能通过其KGD基序干扰HAdV-5对细胞整联蛋白的吸附，而葡萄球菌A5 / 80噬菌体不具有此结构。这项研究的目的是研究T4和A5 / 80噬菌体制剂对5型人腺病毒（HAdV-5）DNA合成和HAdV-5基因表达的影响。方法对A549细胞株进行实验。HAdV-5 DNA合成用实时PCR进行了研究。在预孵育和共孵育实验中，通过定量实时PCR确定HAdV-5早期（DBP）和晚期（六邻体）基因的表达。结果虽然两种噬菌体制剂均能显着降低HAdV-5基因的表达，但HAdV-5 DNA的合成仅受T4抑制。结论噬菌体制剂有望作为新型抗病毒药物。但是，需要进一步研究以研究其抗病毒作用。