Holomastigotes is a protist genus (Parabasalia: Spirotrichonymphea) that resides in the hindguts of “lower” termites. It can be distinguished from other parabasalids by spiral flagellar bands that run along the entire length of the cell, an anterior nucleus, a reduced or absent axostyle, the presence of spherical vesicles inside the cells, and the absence of ingested wood particles. Eight species have been described based on their morphology so far, although no molecular data were available prior to this study. We determined the 18S rRNA gene sequences of Holomastigotes from the hindguts of Hodotermopsis sjostedti, Reticulitermes flavipes, Reticulitermes lucifugus, and Reticulitermes tibialis. Phylogenetic analyses placed all sequences in an exclusive and well‐supported clade with the type species, Holomastigotes elongatum from R. lucifugus. However, the phylogenetic position of Holomastigotes within the Spirotrichonymphea was not resolved. We describe two new species, Holomastigotes flavipes n. sp. and Holomastigotes tibialis n. sp., inhabiting the hindguts of R. flavipes and R. tibialis, respectively.

中文翻译：

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Holomastigotes (Spirotrichonymphea, Parabasalia) 的分子特性与 Holomastigotes flavipes n 的描述。sp. 和 Holomastigotes tibialis n。sp

Holomastigotes 是一种原生生物属（Parabasalia：Spirotrichonymphea），位于“低等”白蚁的后肠中。它可以通过沿着细胞的整个长度延伸的螺旋鞭毛带、前核、轴突减少或缺失、细胞内存在球形囊泡以及没有摄入的木颗粒来与其他副基底层动物区分开来。到目前为止，已经根据它们的形态描述了 8 个物种，尽管在这项研究之前没有可用的分子数据。我们从 Hodotermopsis sjostedti、Reticulitermes flavipes、Reticulitermes lucifugus 和 Reticulitermes tibialis 的后肠中确定了 Holomastigotes 的 18S rRNA 基因序列。系统发育分析将所有序列置于具有模式物种的独特且得到充分支持的进化枝中，来自 R. lucifugus 的 Holomastigotes elongatum。然而，Holomastigotes 在 Spirotrichonymphea 中的系统发育位置尚未解决。我们描述了两个新物种 Holomastigotes flavipes n。sp. 和 Holomastigotes tibialis n。sp., 分别栖息在 R. flavipes 和 R. tibialis 的后肠。