BACKGROUND The gene order in a eukaryotic genome is not random. Some neighboring genes show specific similarities, while others become separated during evolution. Whole genome duplication events (WGDs) have been recognized as an important evolutionary force. The potential relationship between the separation of neighboring genes and WGDs needs to be investigated. In this study, we investigated whether there is a potential relationship between separated neighboring gene pairs and WGDs, and the mechanism by which neighboring genes are separated. Additionally, we studied whether neighboring genes tend to show intrachromosomal colocalization after their neighborhood was disrupted and the factors facilitating the intrachromosomal colocalization of separated neighboring genes. RESULTS The separation of neighboring gene pairs is closely related to whole genome duplication events. Furthermore, we found that there is a double linear relationship between separated neighboring genes, total genes, and WGDs. The process of separation of neighboring genes caused by WGDs is also not random but abides by the double linear model. Separated neighboring gene pairs tend to show intrachromosomal colocalization. The conservativism of separated neighboring genes and histone modification facilitate the intrachromosomal colocalization of neighboring genes after their separation. CONCLUSIONS These results provide new insight into the understanding of evolutionary roles of locations and the relationship of neighboring gene pairs with whole genome duplications. Furthermore, understanding the proposed mechanism for intrachromosomal colocalization of separated genes benefits our knowledge of chromosomal interactions in the nucleus.

中文翻译：

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相邻基因分离后与全基因组重复密切相关。

背景技术真核基因组中的基因顺序不是随机的。一些相邻的基因显示出特定的相似性，而另一些在进化过程中分离。全基因组复制事件（WGDs）已被认为是重要的进化力量。相邻基因分离与WGD之间的潜在关系需要研究。在这项研究中，我们调查了分离的相邻基因对和WGD之间是否存在潜在的关系，以及相邻基因分离的机理。另外，我们研究了邻近基因是否在邻域破坏后趋于显示染色体内共定位以及促进分离的邻近基因在染色体内共定位的因素。结果邻近基因对的分离与全基因组复制事件密切相关。此外，我们发现分离的邻近基因，总基因和WGD之间存在双重线性关系。由WGD引起的相邻基因分离过程也不是随机的，而是遵循双重线性模型。分离的邻近基因对倾向于显示染色体内共定位。分离的邻近基因的保守性和组蛋白修饰促进了邻近基因在分离后的染色体内共定位。结论这些结果为理解位置的进化作用以及邻近基因对与全基因组重复之间的关系提供了新的见识。此外，