How organisms perceive and respond to their surroundings is one of the great questions in biology. It is clear that RNA plays key roles in sensing. Cellular and environmental cues that RNA responds to include temperature, ions, metabolites, and biopolymers. Recent advances in next-generation sequencing and in vivo chemical probing have provided unprecedented insights into RNA folding in vivo and genome-wide. Patterns of chemical reactivity have implicated control of gene expression by RNA and aided prediction of RNA structure. Central to these advances has been development of molecular biological and chemical techniques. Key advances are improvements in the quality, cost, and throughput of library preparation; availability of a wider array of chemicals for probing RNA structure in vivo; and robustness and user friendliness of data analysis. Insights from probing transcriptomes and future directions are provided.

中文翻译：

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探测体内和全基因组 RNA 结构的技术开发。


生物体如何感知和响应周围环境是生物学中的重大问题之一。很明显，RNA 在传感中发挥着关键作用。 RNA 响应的细胞和环境线索包括温度、离子、代谢物和生物聚合物。新一代测序和体内化学探测的最新进展为体内和全基因组范围的 RNA 折叠提供了前所未有的见解。化学反应模式暗示了 RNA 对基因表达的控制，并有助于预测 RNA 结构。这些进步的核心是分子生物学和化学技术的发展。主要进步是文库制备的质量、成本和通量的提高；提供更广泛的化学物质来探测体内 RNA 结构；以及数据分析的稳健性和用户友好性。提供了探索转录组的见解和未来的方向。