Effect of environmental factors on production of lichenin, a bacteriocin-like compound produced by Bacillus licheniformis 26L-10/3RA isolated from buffalo rumen was studied. Lichenin represents the first anaerobiosis-specific expression of broad-spectrum antibacterial compound effective only under anaerobic conditions. Production of lichenin by B. licheniformis 26L-10/3RA was found to be very high at 39 degrees C in L-10 medium supplemented with 0.5% glucose and 20% (w/v) inert thermocol beads. Lichenin production was highest at pH 6.8 after 72-96h of incubation. Our study also indicated that Lichenin is not a plasmid-linked characteristic and is encoded by chromosomal DNA. Results obtained can be used in large-scale production of Lichenin for potential application in manipulating rumen function intended for improving productivity of the ruminants.

中文翻译：

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环境因素对地衣芽孢杆菌（地衣芽孢杆菌26L-10 / 3RA产生的一种染色体编码的细菌素样化合物）的产生的影响。

研究了环境因素对地衣藻产量的影响，地衣藻是一种由水牛瘤胃分离的地衣芽孢杆菌26L-10 / 3RA产生的细菌素样化合物。地衣素代表仅在厌氧条件下有效的广谱抗菌化合物的厌氧菌特异性表达。发现地衣芽孢杆菌26L-10 / 3RA在补充了0.5％葡萄糖和20％（w / v）惰性热col珠的L-10培养基中，地衣芽孢杆菌的地衣素产量在39摄氏度时非常高。温育72-96h后，地衣素产量最高，为pH 6.8。我们的研究还表明，地衣素不是质粒连接的特征，而是由染色体DNA编码的。所得结果可用于大规模生产地衣宁，可潜在地用于操纵旨在提高反刍动物生产力的瘤胃功能。