BACKGROUND BM-derived mesenchymal stem cells (MSC) are attractive sources for autotransplantation with no risk of rejection, but the use of these cells bas problems, including the necessity of harvesting BM from donors, the donors' age-dependency, limitation to autologous use and difficulty of use for patients with hereditary diseases. We report a method of isolating placenta-derived mesenchymal progenitor cells (PDMPC) that can be used as an alternative source of MSC. METHODS We isolated PDMPC from human fetal chorionic villi using the explant culture method, from placentas collected after neonatal delivery (38-40 weeks of gestation). The PDMPC were characterized by morphologic and immunophenotypic analysis. The differentiation ability of mesenchymal and neural lineages was detected using specific culture conditions and determined by morphology, reverse transcription(RT)-PCR, histochemical staining and immunocytostaining. RESULTS The PDMPC all originated from fetal chorionic villi, as confirmed by fluorescence in situ hybridization analysis. The PDMPC population consisted of spindle-shaped cells and large flat cells. The PDMPCexpressed CD13, CD44, CD73, CD90, CDIO5 and HLA class I as surface epitopes, but not CD31, CD34, CD45 and HLA-DR. These cells differentiated into osteocytes, chondrocytes and adipocytes under specific culture conditions, and were also induced to form neural-like cells. DISCUSSION Our study shows that PDMPC can differentiate into mesenchymal lineages and be induced to form neural-like cells. Thus, PDMPCisolated from chorionic villi of placenta may provide a novel source for the research of stem and progenitor cells in placenta, cell therapy and regenerative medicine, particularly as a source of allogenic mesenchymal stem and progenitor cells with little ethical conflict and various advantages

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人胎盘绒毛膜间充质祖细胞的分离与表征

背景骨髓间充质干细胞 (MSC) 是有吸引力的自体移植来源，没有排斥风险，但这些细胞的使用存在一些问题，包括必须从捐赠者那里获得骨髓、捐赠者的年龄依赖性、对自体使用的限制和遗传性疾病患者使用困难。我们报告了一种分离胎盘来源间充质祖细胞 (PDMPC) 的方法，该方法可用作 MSC 的替代来源。方法 我们使用外植体培养方法从新生儿分娩后（妊娠 38-40 周）收集的胎盘中分离出 PDMPC。PDMPC 的特点是形态学和免疫表型分析。使用特定培养条件检测间充质和神经谱系的分化能力，并通过形态学、逆转录(RT)-PCR、组织化学染色和免疫细胞染色确定。结果经荧光原位杂交分析证实，PDMPC均来源于胎儿绒毛膜绒毛。PDMPC 群体由纺锤形细胞和大扁平细胞组成。PDMPC 将 CD13、CD44、CD73、CD90、CDIO5 和 HLA I 类表达为表面表位，但不表达 CD31、CD34、CD45 和 HLA-DR。这些细胞在特定的培养条件下分化为骨细胞、软骨细胞和脂肪细胞，并被诱导形成神经样细胞。讨论 我们的研究表明 PDMPC 可以分化为间充质谱系并被诱导形成神经样细胞。因此，