Parietariajudaica L. (Urticaceae) pollen is considered one of the most common causes of allergic respiratory symptoms in the Mediterranean area. The localization of lipid transfer proteins (LTPs) in P. judaica mature and hydrated-activated pollen grains was investigated applying a combination of transmission electron microscopy (TEM) with immunocytochemical methods. Our results show that the content of LTPs in P. judaica pollen grains changes during the process of hydration. The localization of judaica LTPs in the cytoplasm and in the lipid bodies associated with vacuoles demonstrated that LTPs represent primarily intracellular proteins. On the other hand, exposure of the pollen grains to germination medium induced the release of LTPs from the pollen grain. Thus, LTPs are cytoplasmic proteins that are secreted to become available for pollen-stigma interactions and probably induce the IgE antibody responses in allergic patients.

中文翻译：

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Parietaria judaica L.花粉粒中的脂质转移蛋白：免疫细胞化学定位和功能。

Parietariajudaica L.（荨麻科）花粉被认为是地中海地区过敏性呼吸道症状的最常见原因之一。利用透射电子显微镜（TEM）和免疫细胞化学方法相结合，研究了枣假单胞菌成熟和水合活化花粉粒中脂质转移蛋白（LTP）的定位。我们的结果表明，在水合作用过程中，褐紫苏花粉粒中LTP的含量会发生变化。Judaica LTP在细胞质和与液泡相关的脂质体中的定位表明LTP主要代表细胞内蛋白。另一方面，将花粉粒暴露于发芽培养基中会诱导LTP从花粉粒中释放。从而，