The incidence and diversity of enterococci in retail food samples of meat, dairy and vegetable origin was investigated. Enterococci were present, at concentrations of 10(1) to 10(4) CFU/g. Fifty selected isolates from food samples grouped in two separate clusters by RAPD analysis. Cluster G1 (72% of the isolates) contained the E. faecium CECT 410T type strain, and also showed a high degree of genetic diversity. Cluster G2 (28% of the isolates) contained the E. faecalis CECT 481T type strain and was genetically more homogeneous. Virulence traits (haemolysin, gelatinase or DNAse activities, or the presence of structural genes cylL, ace, asal and esp) were not detected. All isolates were sensitive to the antibiotics ampicillin, penicillin, gentamicin, streptomycin and chloramphenicol. A high pecentage of isolates were resistant to erythromycin and rifampicin. Many isolates showed intermediate sensitivity to several antibiotics (tetracycline, ciprofloxacin, levofloxacin, or quinupristin/dalfopristin). Vancomycin and teicoplanin resistance was detected in one strain, but vanA, vanB, vanC1, vanC2 or vanC3 genes were not detected. Many of the isolates showed functional properties of food or health relevance. Production of antimicrobial substances was detected in 17 of the isolates, and 14 of them carried structural genes for enterocins A, B and/or P.

中文翻译：

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从西班牙不同食品中分离出来的肠球菌的功能和安全性方面。

调查了肉类，奶类和蔬菜类零售食品中肠球菌的发生率和多样性。肠球菌的浓度为10（1）至10（4）CFU / g。通过RAPD分析，从食物样品中选择的50种分离物分为两个单独的簇。G1簇（72％的分离株）含有屎肠球菌CECT 410T型菌株，并显示出高度的遗传多样性。G2簇（占分离株的28％）含有粪肠球菌CECT 481T型菌株，遗传上更均一。未检测到毒力特征（溶血素，明胶酶或DNAse活性，或存在结构基因cylL，ace，asal和esp）。所有分离株均对抗生素氨苄西林，青霉素，庆大霉素，链霉素和氯霉素敏感。高分离度的菌株对红霉素和利福平有抗性。许多分离株对几种抗生素（四环素，环丙沙星，左氧氟沙星或奎奴普丁/达福普汀）具有中等敏感性。在一个菌株中检测到万古霉素和替考拉宁耐药，但未检测到vanA，vanB，vanC1，vanC2或vanC3基因。许多分离株显示出与食品或健康相关的功能特性。在其中的17种分离物中检测到了抗菌物质的产生，其中14种带有肠毒素A，B和/或P的结构基因。许多分离株显示出与食品或健康相关的功能特性。在其中的17种分离物中检测到了抗菌物质的产生，其中14种带有肠毒素A，B和/或P的结构基因。许多分离株显示出与食品或健康相关的功能特性。在其中的17种分离物中检测到了抗菌物质的产生，其中14种带有肠毒素A，B和/或P的结构基因。